| pubmed-article:10640296 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10640296 | lifeskim:mentions | umls-concept:C0006865 | lld:lifeskim |
| pubmed-article:10640296 | lifeskim:mentions | umls-concept:C0035820 | lld:lifeskim |
| pubmed-article:10640296 | lifeskim:mentions | umls-concept:C0085828 | lld:lifeskim |
| pubmed-article:10640296 | lifeskim:mentions | umls-concept:C0288011 | lld:lifeskim |
| pubmed-article:10640296 | lifeskim:mentions | umls-concept:C0021467 | lld:lifeskim |
| pubmed-article:10640296 | lifeskim:mentions | umls-concept:C1862324 | lld:lifeskim |
| pubmed-article:10640296 | lifeskim:mentions | umls-concept:C0040649 | lld:lifeskim |
| pubmed-article:10640296 | lifeskim:mentions | umls-concept:C0879590 | lld:lifeskim |
| pubmed-article:10640296 | lifeskim:mentions | umls-concept:C0021469 | lld:lifeskim |
| pubmed-article:10640296 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:10640296 | pubmed:dateCreated | 2000-2-22 | lld:pubmed |
| pubmed-article:10640296 | pubmed:abstractText | We previously reported that immunosuppressive cannabinoids inhibited interleukin (IL)-2 steady-state mRNA expression and secretion by phorbol-12-myristate-13-acetate plus ionomycin-activated mouse splenocytes and EL4 murine T-cells. Here we show that inhibition of IL-2 production by cannabinol, a modest central nervous system-active cannabinoid, is mediated through the inhibition of IL-2 gene transcription. Moreover, electrophoretic mobility shift assays demonstrated that cannabinol markedly inhibited the DNA binding activity of nuclear factor of activated T-cells (NF-AT) and activator protein-1 (AP-1) in a time- and concentration-dependent manner in activated EL4 cells. The inhibitory effects produced by cannabinol on AP-1 DNA binding were quite transient, showing partial recovery by 240 min after cell activation and no effect on the activity of a reporter gene under the control of AP-1. Conversely, cannabinol-mediated inhibition of NF-AT was robust and sustained as demonstrated by an NF-AT-regulated reporter gene. Collectively, these results suggest that decreased IL-2 production by cannabinol in EL4 cells is due to the inhibition of transcriptional activation of the IL-2 gene and is mediated, at least in part, through a transient inhibition of AP-1 and a sustained inhibition of NF-AT. | lld:pubmed |
| pubmed-article:10640296 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10640296 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10640296 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10640296 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10640296 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10640296 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10640296 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10640296 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10640296 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10640296 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10640296 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10640296 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10640296 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10640296 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:10640296 | pubmed:issn | 0022-3565 | lld:pubmed |
| pubmed-article:10640296 | pubmed:author | pubmed-author:RaoL GLG | lld:pubmed |
| pubmed-article:10640296 | pubmed:author | pubmed-author:YangK HKH | lld:pubmed |
| pubmed-article:10640296 | pubmed:author | pubmed-author:KaminskiN ENE | lld:pubmed |
| pubmed-article:10640296 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10640296 | pubmed:volume | 292 | lld:pubmed |
| pubmed-article:10640296 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10640296 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10640296 | pubmed:pagination | 597-605 | lld:pubmed |
| pubmed-article:10640296 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:10640296 | pubmed:meshHeading | pubmed-meshheading:10640296... | lld:pubmed |
| pubmed-article:10640296 | pubmed:meshHeading | pubmed-meshheading:10640296... | lld:pubmed |
| pubmed-article:10640296 | pubmed:meshHeading | pubmed-meshheading:10640296... | lld:pubmed |
| pubmed-article:10640296 | pubmed:meshHeading | pubmed-meshheading:10640296... | lld:pubmed |
| pubmed-article:10640296 | pubmed:meshHeading | pubmed-meshheading:10640296... | lld:pubmed |
| pubmed-article:10640296 | pubmed:meshHeading | pubmed-meshheading:10640296... | lld:pubmed |
| pubmed-article:10640296 | pubmed:meshHeading | pubmed-meshheading:10640296... | lld:pubmed |
| pubmed-article:10640296 | pubmed:meshHeading | pubmed-meshheading:10640296... | lld:pubmed |
| pubmed-article:10640296 | pubmed:meshHeading | pubmed-meshheading:10640296... | lld:pubmed |
| pubmed-article:10640296 | pubmed:meshHeading | pubmed-meshheading:10640296... | lld:pubmed |
| pubmed-article:10640296 | pubmed:meshHeading | pubmed-meshheading:10640296... | lld:pubmed |
| pubmed-article:10640296 | pubmed:meshHeading | pubmed-meshheading:10640296... | lld:pubmed |
| pubmed-article:10640296 | pubmed:meshHeading | pubmed-meshheading:10640296... | lld:pubmed |
| pubmed-article:10640296 | pubmed:meshHeading | pubmed-meshheading:10640296... | lld:pubmed |
| pubmed-article:10640296 | pubmed:meshHeading | pubmed-meshheading:10640296... | lld:pubmed |
| pubmed-article:10640296 | pubmed:meshHeading | pubmed-meshheading:10640296... | lld:pubmed |
| pubmed-article:10640296 | pubmed:meshHeading | pubmed-meshheading:10640296... | lld:pubmed |
| pubmed-article:10640296 | pubmed:meshHeading | pubmed-meshheading:10640296... | lld:pubmed |
| pubmed-article:10640296 | pubmed:meshHeading | pubmed-meshheading:10640296... | lld:pubmed |
| pubmed-article:10640296 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:10640296 | pubmed:articleTitle | Role of nuclear factor of activated T-cells and activator protein-1 in the inhibition of interleukin-2 gene transcription by cannabinol in EL4 T-cells. | lld:pubmed |
| pubmed-article:10640296 | pubmed:affiliation | Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Taejon, Korea. | lld:pubmed |
| pubmed-article:10640296 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10640296 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10640296 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10640296 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10640296 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10640296 | lld:pubmed |
