| pubmed-article:10625396 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10625396 | lifeskim:mentions | umls-concept:C0004651 | lld:lifeskim |
| pubmed-article:10625396 | lifeskim:mentions | umls-concept:C0004611 | lld:lifeskim |
| pubmed-article:10625396 | lifeskim:mentions | umls-concept:C0034865 | lld:lifeskim |
| pubmed-article:10625396 | lifeskim:mentions | umls-concept:C0023621 | lld:lifeskim |
| pubmed-article:10625396 | lifeskim:mentions | umls-concept:C0003241 | lld:lifeskim |
| pubmed-article:10625396 | lifeskim:mentions | umls-concept:C0205171 | lld:lifeskim |
| pubmed-article:10625396 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:10625396 | pubmed:dateCreated | 2000-2-29 | lld:pubmed |
| pubmed-article:10625396 | pubmed:abstractText | The creation of large phage antibody libraries has become an important goal in selecting antibodies against any antigen. Here we describe a method for making libraries so large that the complete diversity cannot be accessed using traditional phage technology. This involves the creation of a primary phage scFv library in a phagemid vector containing two nonhomologous lox sites. Contrary to the current dogma, we found that infecting Cre recombinase-expressing bacteria by such a primary library at a high multiplicity of infection results in the entry of many different phagemid into the cell. Exchange of Vh and Vl genes between such phagemids creates many new V h/Vl combinations, all of which are functional. On the basis of the observed recombination, the library is calculated to have a diversity of 3x1011. A library created using this method was validated by the selection of high affinity antibodies against a large number of different protein antigens. | lld:pubmed |
| pubmed-article:10625396 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10625396 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10625396 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:10625396 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10625396 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10625396 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:10625396 | pubmed:issn | 1087-0156 | lld:pubmed |
| pubmed-article:10625396 | pubmed:author | pubmed-author:BradburyAA | lld:pubmed |
| pubmed-article:10625396 | pubmed:author | pubmed-author:SblatteroDD | lld:pubmed |
| pubmed-article:10625396 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10625396 | pubmed:volume | 18 | lld:pubmed |
| pubmed-article:10625396 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10625396 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10625396 | pubmed:pagination | 75-80 | lld:pubmed |
| pubmed-article:10625396 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
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| pubmed-article:10625396 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:10625396 | pubmed:articleTitle | Exploiting recombination in single bacteria to make large phage antibody libraries. | lld:pubmed |
| pubmed-article:10625396 | pubmed:affiliation | International School for Advanced Studies (SISSA), Biophysics Sector, Via Beirut 2-4, Trieste, 34014, Italy. | lld:pubmed |
| pubmed-article:10625396 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10625396 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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