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pubmed-article:10609320pubmed:abstractTextChanges in the level of lipid free-radical oxidation in mitochondrial fraction at the presence of 20-hydroxyecdysone in 1, 2, 4, 6, 8 microM concentration with the help of a chemiluminescence (ChL) method were investigated in vitro. Statistically authentic reduction of ChL kinetic parameters--I(s) and tg alpha, at 20-hydroxyecdysone presence in concentration of 2 microM was found. 20-hydroxyecdysone administration in concentration 4 microM and more leads to the statistically authentic change of all four ChL parameters. The antioxidizing effect of 20-hydroxyecdysone was compared with action of such antioxidant as a hydroquinone. The higher activity of 20-hydroxyecdysone as an antioxidant in comparison with the hydroquinone was shown. For changes of all four measured kinetic parameters of ChL, concentration of the hydroquinone as much 2-fold than for 20-hydroxyecdysone is necessary. On the basis of our researches in the model system a conclusion was made that 20-hydroxyecdysone has an antioxidizing action on lipid free radical oxidation in mitochondrial fraction in dependents on concentration. 20-Hydroxyecdysone has antioxidizing properties directly, in these conditions in vitro, when its metabolites formation does not occur yet.lld:pubmed
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pubmed-article:10609320pubmed:authorpubmed-author:MorozovaR PRPlld:pubmed
pubmed-article:10609320pubmed:authorpubmed-author:Kuz'menkoA...lld:pubmed
pubmed-article:10609320pubmed:authorpubmed-author:NikolenkoI...lld:pubmed
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pubmed-article:10609320pubmed:pagination35-8lld:pubmed
pubmed-article:10609320pubmed:dateRevised2011-11-17lld:pubmed
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pubmed-article:10609320pubmed:articleTitle[Antioxidant effect of 20-hydroxyecdysone in a model system].lld:pubmed
pubmed-article:10609320pubmed:affiliationA.V. Palladin Institute of Biochemistry of National Academy of Sci, Kyiv.lld:pubmed
pubmed-article:10609320pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:10609320pubmed:publicationTypeEnglish Abstractlld:pubmed