| pubmed-article:10604971 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10604971 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:10604971 | lifeskim:mentions | umls-concept:C0012984 | lld:lifeskim |
| pubmed-article:10604971 | lifeskim:mentions | umls-concept:C0022646 | lld:lifeskim |
| pubmed-article:10604971 | lifeskim:mentions | umls-concept:C0442805 | lld:lifeskim |
| pubmed-article:10604971 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:10604971 | lifeskim:mentions | umls-concept:C0078437 | lld:lifeskim |
| pubmed-article:10604971 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:10604971 | pubmed:dateCreated | 2000-1-27 | lld:pubmed |
| pubmed-article:10604971 | pubmed:abstractText | The effect of W-7 [N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide hydrochloride] on Ca(2+) signaling in Madin-Darby canine kidney cells was investigated. W-7 (0.1-1 mM) induced a [Ca(2+)](i) increase, which comprised an initial increase and a plateau. Ca(2+) removal inhibited the Ca(2+) signals by 80%, suggesting that W-7 activated external Ca(2+) influx and internal Ca(2+) release. Pretreatment with the mitochondrial uncoupler carbonylcyanide m-chlorophenylhydrazone (2 microM) and the endoplasmic reticulum Ca(2+) pump inhibitor thapsigargin (1 microM) abolished the internal Ca(2+) release induced by 0.5 mM W-7; conversely, pretreatment with W-7 prevented thapsigargin and carbonylcyanide m-chlorophenylhydrazone from releasing internal Ca(2+). W-7 (0.2 mM) induced Mn(2+) quench of fura-2 fluorescence, which was inhibited by La(3+) (0.1 mM) by 80%. La(3+) (0.1 mM) partly inhibited 0.2 mM W-7-induced [Ca(2+)](i) increase. Addition of 5 mM Ca(2+) induced a significant [Ca(2+)](i) increase after pretreating with 0.2 to 1 mM W-7 in Ca(2+)-free medium for 5 min, suggesting that W-7 induced capacitative Ca(2+) entry. W-7 (0.5 mM) potentiated the capacitative Ca(2+) entry induced by 1 microM thapsigargin by 15%. Pretreatment with aristolochic acid (40 microM) to inhibit phospholipase A(2) reduced 0.5 mM W-7-induced internal Ca(2+) release and external Ca(2+) influx by 25 and 80%, respectively. Inhibition of phospholipase C with U73122 (2 microM) or inhibition of phospholipase D with propranolol (0.1 mM) had no effect on the internal Ca(2+) release induced by 0.5 mM W-7. It remains unclear whether W-7 induced [Ca(2+)](i) increases via inhibition of calmodulin. Three other calmodulin inhibitors (phenoxybenzamine, trifluoperazine, and fluphenazine-N-chloroethane) did not alter resting [Ca(2+)](i). | lld:pubmed |
| pubmed-article:10604971 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10604971 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10604971 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10604971 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10604971 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10604971 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10604971 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10604971 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10604971 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10604971 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10604971 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10604971 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10604971 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10604971 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10604971 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10604971 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:10604971 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10604971 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10604971 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:10604971 | pubmed:issn | 0022-3565 | lld:pubmed |
| pubmed-article:10604971 | pubmed:author | pubmed-author:TsengC JCJ | lld:pubmed |
| pubmed-article:10604971 | pubmed:author | pubmed-author:JanC RCR | lld:pubmed |
| pubmed-article:10604971 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10604971 | pubmed:volume | 292 | lld:pubmed |
| pubmed-article:10604971 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10604971 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10604971 | pubmed:pagination | 358-65 | lld:pubmed |
| pubmed-article:10604971 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:10604971 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:10604971 | pubmed:articleTitle | W-7 induces [Ca(2+)](i) increases in Madin-Darby canine kidney (MDCK) cells. | lld:pubmed |
| pubmed-article:10604971 | pubmed:affiliation | Department of Medical Education and Research, Veterans General Hospital-Kaohsiung, Taiwan, Republic of China. crjan@isca.vghks.gov.tw | lld:pubmed |
| pubmed-article:10604971 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10604971 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10604971 | lld:pubmed |
