| pubmed-article:106042 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:106042 | lifeskim:mentions | umls-concept:C0004611 | lld:lifeskim |
| pubmed-article:106042 | lifeskim:mentions | umls-concept:C0035503 | lld:lifeskim |
| pubmed-article:106042 | lifeskim:mentions | umls-concept:C0028174 | lld:lifeskim |
| pubmed-article:106042 | lifeskim:mentions | umls-concept:C0376315 | lld:lifeskim |
| pubmed-article:106042 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:106042 | pubmed:dateCreated | 1979-5-23 | lld:pubmed |
| pubmed-article:106042 | pubmed:abstractText | Acetylene reduction by nitrogenase from Rhodospirillum rubrum, unlike that by other nitrogenases, was recently found by other investigators to require an activation of the iron protein of nitrogenase by an activating system comprising a chromatophore membrane component, adenosine 5'-triphosphate (ATP), and divalent metal ions. In an extension of this work, we observed that the same activating system was also required for nitrogenase-linked H(2) evolution. However, we found that, depending on their nitrogen nutrition regime, R. rubrum cells produced two forms of nitrogenase that differed in their Fe protein components. Cells whose nitrogen supply was totally exhausted before harvest yielded predominantly a form of nitrogenase (A) whose enzymatic activity was not governed by the activating system, whereas cells supplied up to harvest time with N(2) or glutamate yielded predominantly a form of nitrogenase (R) whose enzymatic activity was regulated by the activating system. An unexpected finding was the rapid (less than 10 min in some cases) intracellular conversion of nitrogenase A to nitrogenase R brought about by the addition to nitrogen-starved cells of glutamine, asparagine, or, particularly, ammonia. This finding suggests that mechanisms other than de novo protein synthesis were involved in the conversion of nitrogenase A to the R form. The molecular weights of the Fe protein and Mo-Fe protein components from nitrogenases A and R were the same. However, nitrogenase A appeared to be larger in size, because it had more Fe protein units per Mo-Fe protein than did nitrogenase R. A distinguishing property of the Fe protein from nitrogenase R was its ATP requirement. When combined with the Mo-Fe protein (from either nitrogenase A or nitrogenase R), the R form of Fe protein required a lower ATP concentration but bound or utilized more ATP molecules during acetylene reduction than did the A form of Fe protein. No differences between the Fe proteins from the two forms of nitrogenase were found in the electron paramagnetic resonance spectrum, midpoint oxidation-reduction potential, or sensitivity to iron chelators. | lld:pubmed |
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| pubmed-article:106042 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:106042 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:106042 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:106042 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:106042 | pubmed:issn | 0021-9193 | lld:pubmed |
| pubmed-article:106042 | pubmed:author | pubmed-author:ArnonD IDI | lld:pubmed |
| pubmed-article:106042 | pubmed:author | pubmed-author:ZUTZH UHU | lld:pubmed |
| pubmed-article:106042 | pubmed:author | pubmed-author:CarithersR... | lld:pubmed |
| pubmed-article:106042 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:106042 | pubmed:volume | 137 | lld:pubmed |
| pubmed-article:106042 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:106042 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:106042 | pubmed:pagination | 779-89 | lld:pubmed |
| pubmed-article:106042 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:106042 | pubmed:year | 1979 | lld:pubmed |
| pubmed-article:106042 | pubmed:articleTitle | Two forms of nitrogenase from the photosynthetic bacterium Rhodospirillum rubrum. | lld:pubmed |
| pubmed-article:106042 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:106042 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
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