| pubmed-article:10578114 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10578114 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:10578114 | lifeskim:mentions | umls-concept:C0021585 | lld:lifeskim |
| pubmed-article:10578114 | lifeskim:mentions | umls-concept:C0043117 | lld:lifeskim |
| pubmed-article:10578114 | lifeskim:mentions | umls-concept:C1171362 | lld:lifeskim |
| pubmed-article:10578114 | lifeskim:mentions | umls-concept:C0027895 | lld:lifeskim |
| pubmed-article:10578114 | lifeskim:mentions | umls-concept:C0030956 | lld:lifeskim |
| pubmed-article:10578114 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:10578114 | lifeskim:mentions | umls-concept:C1515670 | lld:lifeskim |
| pubmed-article:10578114 | lifeskim:mentions | umls-concept:C0162585 | lld:lifeskim |
| pubmed-article:10578114 | lifeskim:mentions | umls-concept:C0936012 | lld:lifeskim |
| pubmed-article:10578114 | lifeskim:mentions | umls-concept:C0998392 | lld:lifeskim |
| pubmed-article:10578114 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:10578114 | pubmed:dateCreated | 1999-12-28 | lld:pubmed |
| pubmed-article:10578114 | pubmed:abstractText | We have produced an active form of Schistocerca gregaria ion transport peptide (ITP) in an insect cell expression system. Transformed Drosophila Kc1 cells secreted a form of ITP into the cell culture medium that was proteolytically cleaved correctly at the amino (N)-terminus. Concentrated culture supernatant from transformed Kc1 and Hi5 cells had high biological activity when tested on isolated locust ilea. Conversely, ITP expressed by baculovirus-infected Sf9 cells was larger in size and had decreased specific activity compared to ITP produced by Kc1 cells due to incorrect cleavage of the peptide at the N-terminus in the baculovirus system. This demonstrates how processing of the secreted foreign protein (ITP) expressed under the late polyhedrin promoter is compromised in a baculovirus-infected cell. Transient transformation of Kc1 cells results in supernatants containing two forms of ITP; one form (A) co-elutes with synthetic ITP and the other form (B) has reduced electrophoretic mobility. In contrast, in stably transformed Kc1 cell supernatant, ITP is expressed in a single form, which has the same electrophoretic mobility and specific biological activity as form A produced by transiently transformed Kc1 cells. Arch. | lld:pubmed |
| pubmed-article:10578114 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10578114 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10578114 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10578114 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10578114 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10578114 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10578114 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10578114 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10578114 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:10578114 | pubmed:issn | 0739-4462 | lld:pubmed |
| pubmed-article:10578114 | pubmed:author | pubmed-author:WangY JYJ | lld:pubmed |
| pubmed-article:10578114 | pubmed:author | pubmed-author:PhillipsJ EJE | lld:pubmed |
| pubmed-article:10578114 | pubmed:author | pubmed-author:GrigliattiT... | lld:pubmed |
| pubmed-article:10578114 | pubmed:author | pubmed-author:ZhaiSS | lld:pubmed |
| pubmed-article:10578114 | pubmed:author | pubmed-author:BrockH WHW | lld:pubmed |
| pubmed-article:10578114 | pubmed:author | pubmed-author:MeredithJJ | lld:pubmed |
| pubmed-article:10578114 | pubmed:author | pubmed-author:TheilmannD... | lld:pubmed |
| pubmed-article:10578114 | pubmed:author | pubmed-author:PfeiferT ATA | lld:pubmed |
| pubmed-article:10578114 | pubmed:author | pubmed-author:HegedusDD | lld:pubmed |
| pubmed-article:10578114 | pubmed:copyrightInfo | Copyright 1999 Wiley-Liss, Inc. | lld:pubmed |
| pubmed-article:10578114 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10578114 | pubmed:volume | 42 | lld:pubmed |
| pubmed-article:10578114 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10578114 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10578114 | pubmed:pagination | 245-52 | lld:pubmed |
| pubmed-article:10578114 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:10578114 | pubmed:meshHeading | pubmed-meshheading:10578114... | lld:pubmed |
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| pubmed-article:10578114 | pubmed:meshHeading | pubmed-meshheading:10578114... | lld:pubmed |
| pubmed-article:10578114 | pubmed:meshHeading | pubmed-meshheading:10578114... | lld:pubmed |
| pubmed-article:10578114 | pubmed:meshHeading | pubmed-meshheading:10578114... | lld:pubmed |
| pubmed-article:10578114 | pubmed:meshHeading | pubmed-meshheading:10578114... | lld:pubmed |
| pubmed-article:10578114 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10578114 | pubmed:articleTitle | Analysis of an insect neuropeptide, Schistocerca gregaria ion transport peptide (ITP), expressed in insect cell systems. | lld:pubmed |
| pubmed-article:10578114 | pubmed:affiliation | Department of Zoology, University of British Columbia, Vancouver, BC V6T1Z4 Canada. | lld:pubmed |
| pubmed-article:10578114 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10578114 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
