| pubmed-article:10562330 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10562330 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:10562330 | lifeskim:mentions | umls-concept:C0025914 | lld:lifeskim |
| pubmed-article:10562330 | lifeskim:mentions | umls-concept:C0026809 | lld:lifeskim |
| pubmed-article:10562330 | lifeskim:mentions | umls-concept:C0036536 | lld:lifeskim |
| pubmed-article:10562330 | lifeskim:mentions | umls-concept:C0036537 | lld:lifeskim |
| pubmed-article:10562330 | lifeskim:mentions | umls-concept:C0687028 | lld:lifeskim |
| pubmed-article:10562330 | lifeskim:mentions | umls-concept:C0007776 | lld:lifeskim |
| pubmed-article:10562330 | lifeskim:mentions | umls-concept:C0022646 | lld:lifeskim |
| pubmed-article:10562330 | lifeskim:mentions | umls-concept:C0022655 | lld:lifeskim |
| pubmed-article:10562330 | lifeskim:mentions | umls-concept:C0001613 | lld:lifeskim |
| pubmed-article:10562330 | lifeskim:mentions | umls-concept:C1257901 | lld:lifeskim |
| pubmed-article:10562330 | lifeskim:mentions | umls-concept:C0596019 | lld:lifeskim |
| pubmed-article:10562330 | lifeskim:mentions | umls-concept:C1516698 | lld:lifeskim |
| pubmed-article:10562330 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:10562330 | lifeskim:mentions | umls-concept:C0539005 | lld:lifeskim |
| pubmed-article:10562330 | lifeskim:mentions | umls-concept:C0539006 | lld:lifeskim |
| pubmed-article:10562330 | pubmed:dateCreated | 2000-2-14 | lld:pubmed |
| pubmed-article:10562330 | pubmed:abstractText | 1. Using RT-PCR, Northern blot analysis, and immunocytochemistry, we confirmed renal expression of proteinase-activated receptor (PAR-2) and demonstrated its presence in native renal epithelial and in cultured M-1 mouse cortical collecting duct (CCD) cells. 2. We investigated the effects of a PAR-2 activating peptide (AP), corresponding to the tethered ligand that is exposed upon trypsin cleavage, and of trypsin on M-1 cells using patch-clamp, intracellular calcium (fura-2) and transepithelial short-circuit current (ISC) measurements. 3. In single M-1 cells, addition of AP elicited a concentration-dependent transient increase in the whole-cell conductance. Removal of extracellular Na+ had no effect while removal of Cl- prevented the stimulation of outward currents. The intracellular calcium concentration increased significantly upon application of AP while a Ca2+-free pipette solution completely abolished the electrical response to AP. 4. In confluent monolayers of M-1 cells, apical application of AP had no effect on ISC whereas subsequent basolateral application elicited a transient increase in ISC. This increase was not due to a stimulation of electrogenic Na+ absorption since the response was preserved in the presence of amiloride. 5. The ISC response to AP was reduced in the presence of the Cl- channel blocker diphenylamine-2-carboxylic acid on the apical side and abolished in the absence of extracellular Cl-. 6. Trypsin elicited similar responses to those to AP while application of a peptide (RP) with the reverse amino acid sequence of AP had no effect on whole-cell currents or ISC. 7. In conclusion, our data suggest that AP or trypsin stimulates Cl- secretion by Ca2+-activated Cl- channels in M-1 CCD cells by activating basolateral PAR-2. | lld:pubmed |
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| pubmed-article:10562330 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10562330 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10562330 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10562330 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:10562330 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10562330 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:10562330 | pubmed:issn | 0022-3751 | lld:pubmed |
| pubmed-article:10562330 | pubmed:author | pubmed-author:FrömterEE | lld:pubmed |
| pubmed-article:10562330 | pubmed:author | pubmed-author:BunnettN WNW | lld:pubmed |
| pubmed-article:10562330 | pubmed:author | pubmed-author:SteinhoffMM | lld:pubmed |
| pubmed-article:10562330 | pubmed:author | pubmed-author:KorbmacherCC | lld:pubmed |
| pubmed-article:10562330 | pubmed:author | pubmed-author:KongWW | lld:pubmed |
| pubmed-article:10562330 | pubmed:author | pubmed-author:LethPP | lld:pubmed |
| pubmed-article:10562330 | pubmed:author | pubmed-author:HigginsM AMA | lld:pubmed |
| pubmed-article:10562330 | pubmed:author | pubmed-author:BertogMM | lld:pubmed |
| pubmed-article:10562330 | pubmed:author | pubmed-author:Bielfeld-Acke... | lld:pubmed |
| pubmed-article:10562330 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10562330 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:10562330 | pubmed:volume | 521 Pt 1 | lld:pubmed |
| pubmed-article:10562330 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10562330 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10562330 | pubmed:pagination | 3-17 | lld:pubmed |
| pubmed-article:10562330 | pubmed:dateRevised | 2010-8-25 | lld:pubmed |
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