| pubmed-article:10550773 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10550773 | lifeskim:mentions | umls-concept:C0262950 | lld:lifeskim |
| pubmed-article:10550773 | lifeskim:mentions | umls-concept:C0441655 | lld:lifeskim |
| pubmed-article:10550773 | lifeskim:mentions | umls-concept:C1533691 | lld:lifeskim |
| pubmed-article:10550773 | lifeskim:mentions | umls-concept:C1515655 | lld:lifeskim |
| pubmed-article:10550773 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:10550773 | pubmed:dateCreated | 1999-12-28 | lld:pubmed |
| pubmed-article:10550773 | pubmed:abstractText | Recombinant human bone morphogenetic protein-2 (rhBMP-2) was biotinated, and the bioactivity of biotinated protein was assessed in vitro (alkaline phosphate induction in limb bud cells) and in vivo (osteoinduction in the rat ectopic assay). Amino-biotinated rhBMP-2 exhibited an increase in bioactivity whereas carboxy-biotinated rhBMP-2 did not exhibit any changes in bioactivity in vitro. Avidin inhibited the bioactivity of amino-biotinated but not carboxyl-biotinated rhBMP-2. Both amino- and carboxy-modified rhBMP-2 induced bone at an equivalent level to that of unmodified rhBMP-2 in vivo. The presence of avidin did not affect the osteoinductive activity of both types of biotinated rhBMP-2. The overall results indicated that binding to a large protein, avidin, might affect rhBMP-2 activity in vitro depending on the binding site; however, in vivo activity was unaffected by the avidin binding. | lld:pubmed |
| pubmed-article:10550773 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10550773 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10550773 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10550773 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10550773 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10550773 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10550773 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10550773 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10550773 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10550773 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10550773 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10550773 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10550773 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10550773 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:10550773 | pubmed:issn | 0006-3592 | lld:pubmed |
| pubmed-article:10550773 | pubmed:author | pubmed-author:GoldenJJ | lld:pubmed |
| pubmed-article:10550773 | pubmed:author | pubmed-author:PalmerRR | lld:pubmed |
| pubmed-article:10550773 | pubmed:author | pubmed-author:WozneyJ MJM | lld:pubmed |
| pubmed-article:10550773 | pubmed:author | pubmed-author:UludagHH | lld:pubmed |
| pubmed-article:10550773 | pubmed:copyrightInfo | Copyright 1999 John Wiley & Sons, Inc. | lld:pubmed |
| pubmed-article:10550773 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10550773 | pubmed:day | 20 | lld:pubmed |
| pubmed-article:10550773 | pubmed:volume | 65 | lld:pubmed |
| pubmed-article:10550773 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10550773 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10550773 | pubmed:pagination | 668-72 | lld:pubmed |
| pubmed-article:10550773 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
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| pubmed-article:10550773 | pubmed:meshHeading | pubmed-meshheading:10550773... | lld:pubmed |
| pubmed-article:10550773 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10550773 | pubmed:articleTitle | Biotinated bone morphogenetic protein-2: In vivo and in vitro activity. | lld:pubmed |
| pubmed-article:10550773 | pubmed:affiliation | Genetics Institute, Bone Biology and Application, One Burtt Road, Andover, Massachusetts 01810, USA. hasan.uludag@yakberta.ca | lld:pubmed |
| pubmed-article:10550773 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10550773 | pubmed:publicationType | Comparative Study | lld:pubmed |
