| pubmed-article:10516278 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10516278 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:10516278 | lifeskim:mentions | umls-concept:C0332307 | lld:lifeskim |
| pubmed-article:10516278 | lifeskim:mentions | umls-concept:C0013935 | lld:lifeskim |
| pubmed-article:10516278 | lifeskim:mentions | umls-concept:C0553257 | lld:lifeskim |
| pubmed-article:10516278 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:10516278 | lifeskim:mentions | umls-concept:C1419072 | lld:lifeskim |
| pubmed-article:10516278 | lifeskim:mentions | umls-concept:C1418759 | lld:lifeskim |
| pubmed-article:10516278 | lifeskim:mentions | umls-concept:C1456395 | lld:lifeskim |
| pubmed-article:10516278 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:10516278 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:10516278 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:10516278 | lifeskim:mentions | umls-concept:C2811999 | lld:lifeskim |
| pubmed-article:10516278 | pubmed:issue | 4 Pt 2 | lld:pubmed |
| pubmed-article:10516278 | pubmed:dateCreated | 1999-11-17 | lld:pubmed |
| pubmed-article:10516278 | pubmed:abstractText | The aim of the present study was to characterize the type(s) of NaPO(4) cotransporter expressed in the human renal cell line HEK-293 and its regulation by parathyroid hormone (PTH) in wild-type cells and in cells transfected by the PTH/PTH-related protein (PTHrP) receptor. The results showed that human embryonic kidney HEK-293 cells expressed NaPO(4) cotransporter type III (PiT1) mRNA and protein. In contrast, type I (NPT1) or II (NPT2) cotransporter mRNA were not expressed. Na(+)-dependent phosphate uptake followed a Michaelis-Menten model (apparent maximal transport rate and affinity constant: 23.32 +/- 0.69 nmol PO(4). mg protein(-1). 10 min(-1) and 0.147 +/- 0.014 mM KH(2)PO(4), respectively), was stimulated by phosphate deprivation (maximal increase 24.5 +/- 0.8%, P < 0.001, after 15 h of phosphate deprivation), and was inhibited by increasing pH (3.6 +/- 0.2-fold decrease at pH 8.5, P < 0.0001). It was inhibited in a time- and concentration-dependent fashion by PTH in HEK-293 cells stably transfected by PTH/PTHrP receptors but not in parental HEK-293 cells. Maximal inhibition of Na(+)-dependent phosphate transport was observed at 30 min after the addition of 72 nM PTH-(1-34) (31.5 +/- 2.4% inhibition, P < 0.01). PTH inhibition of phosphate transport was maintained in phosphate-deprived cells and reversed by both GF109203X (10(-6) M) or staurosporine (5.5 nM), two protein kinase C inhibitors. Na(+)-dependent phosphate uptake was also significantly inhibited by phorbol 12-myristate 13-acetate (20.9 +/- 3.9% inhibition, P < 0.001) but not by dibutyril-cAMP (10(-4) M) or forskolin (50 microM). The physiological role played by type III NaPO(4) cotransport expression in the overall renal regulation of phosphate homeostasis remains to be established. | lld:pubmed |
| pubmed-article:10516278 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10516278 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10516278 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10516278 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10516278 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10516278 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10516278 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10516278 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10516278 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10516278 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10516278 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10516278 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10516278 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10516278 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10516278 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:10516278 | pubmed:issn | 0002-9513 | lld:pubmed |
| pubmed-article:10516278 | pubmed:author | pubmed-author:FernandezRR | lld:pubmed |
| pubmed-article:10516278 | pubmed:author | pubmed-author:FriedlanderGG | lld:pubmed |
| pubmed-article:10516278 | pubmed:author | pubmed-author:SilvaGG | lld:pubmed |
| pubmed-article:10516278 | pubmed:author | pubmed-author:BéliveauRR | lld:pubmed |
| pubmed-article:10516278 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10516278 | pubmed:volume | 277 | lld:pubmed |
| pubmed-article:10516278 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10516278 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10516278 | pubmed:pagination | F543-51 | lld:pubmed |
| pubmed-article:10516278 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
| pubmed-article:10516278 | pubmed:meshHeading | pubmed-meshheading:10516278... | lld:pubmed |
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| pubmed-article:10516278 | pubmed:meshHeading | pubmed-meshheading:10516278... | lld:pubmed |
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| pubmed-article:10516278 | pubmed:meshHeading | pubmed-meshheading:10516278... | lld:pubmed |
| pubmed-article:10516278 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10516278 | pubmed:articleTitle | NaPO(4) cotransport type III (PiT1) expression in human embryonic kidney cells and regulation by PTH. | lld:pubmed |
| pubmed-article:10516278 | pubmed:affiliation | Institut National de la Santé et de la Recherche Médicale, Faculté de Médecine Xavier Bichat, 75018 Paris, France. | lld:pubmed |
| pubmed-article:10516278 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10516278 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
