Manganese peroxidase (MnP) production was performed in a airlift bioreactor in which Phanerochaete chrysosporium I-1512, an MnP hypersecretory strain, was immobilized on a stainless steel mesh. Production was scaled up from a 2.5-L bench scale to a 100-L bioreactor. The yield of MnP was increased 2-fold and reached 6600 U L(-1). These results indicate the feasibility of MnP production on a medium scale, which promises sufficient MnP availability for its use in pulp bleaching at industrial scale.
Laboratoire de Biotechnologie des Champignons Filamenteux, INRA, Centre d'Enseignement Supérieur en Biotechnologie, ESIL, 163 Avenue de Luminy, Case Postale 925, 13288 Marseille Cedex 09, France.
