| pubmed-article:10477697 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10477697 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:10477697 | lifeskim:mentions | umls-concept:C0026473 | lld:lifeskim |
| pubmed-article:10477697 | lifeskim:mentions | umls-concept:C0023810 | lld:lifeskim |
| pubmed-article:10477697 | lifeskim:mentions | umls-concept:C1819439 | lld:lifeskim |
| pubmed-article:10477697 | lifeskim:mentions | umls-concept:C0301625 | lld:lifeskim |
| pubmed-article:10477697 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:10477697 | pubmed:dateCreated | 1999-9-29 | lld:pubmed |
| pubmed-article:10477697 | pubmed:abstractText | Interleukin-12 (IL-12) is a potent proinflammatory and immunoregulatory cytokine skewing T lymphocytes to express a type 1 cytokine pattern. Optimal expression of IL-12 mRNA and bioactivity in vitro requires specific priming of monocytes by interferon-gamma (IFN-gamma) or granulocyte-macrophage colony-stimulating factor (GM-CSF) before lipopolysaccharide (LPS) stimulation. We show here for the first time that the production of IL-12 by IFN-gamma- or GM-CSF-primed human monocytes can be completely suppressed by preincubation with LPS (from Escherichia coli Serotype 055:B5) for 6 to 24 hours before the priming procedure. A dose-dependent suppression of IL-12p70 was measured on the levels of intracellular cytokine production and cytokine secretion. mRNA studies on the expression of p40 and p35 showed an LPS-induced downregulation of both subunits. The results of several different experimental approaches suggest that IL-12 downregulation was not due to endogenous IL-10, IL-4, prostaglandin E(2) (PGE(2)), tumor necrosis factor-alpha (TNF-alpha), or nitric oxide (NO) production induced by LPS. Moreover, preincubation of monocytes with LPS did not lead to a downregulation of the CD14 antigen, which is an LPS receptor. LPS preincubation in this experimental setting did not result in a general hyporesponsiveness of the monocytes, as IL-6 production as well as IFN-gamma-induced upregulation of CD54 did not decline. Downregulation of IL-12 was not due to changes in mRNA stability. These findings show that the immunoregulatory important cytokine, IL-12, underlies itself a complex regulation. | lld:pubmed |
| pubmed-article:10477697 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10477697 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10477697 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:10477697 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10477697 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10477697 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10477697 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10477697 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10477697 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10477697 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10477697 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:10477697 | pubmed:issn | 0006-4971 | lld:pubmed |
| pubmed-article:10477697 | pubmed:author | pubmed-author:SchmidtPP | lld:pubmed |
| pubmed-article:10477697 | pubmed:author | pubmed-author:KapuLL | lld:pubmed |
| pubmed-article:10477697 | pubmed:author | pubmed-author:WerfelTT | lld:pubmed |
| pubmed-article:10477697 | pubmed:author | pubmed-author:WittmannMM | lld:pubmed |
| pubmed-article:10477697 | pubmed:author | pubmed-author:BegemannGG | lld:pubmed |
| pubmed-article:10477697 | pubmed:author | pubmed-author:LarssonV AVA | lld:pubmed |
| pubmed-article:10477697 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10477697 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:10477697 | pubmed:volume | 94 | lld:pubmed |
| pubmed-article:10477697 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10477697 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10477697 | pubmed:pagination | 1717-26 | lld:pubmed |
| pubmed-article:10477697 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
| pubmed-article:10477697 | pubmed:meshHeading | pubmed-meshheading:10477697... | lld:pubmed |
| pubmed-article:10477697 | pubmed:meshHeading | pubmed-meshheading:10477697... | lld:pubmed |
| pubmed-article:10477697 | pubmed:meshHeading | pubmed-meshheading:10477697... | lld:pubmed |
| pubmed-article:10477697 | pubmed:meshHeading | pubmed-meshheading:10477697... | lld:pubmed |
| pubmed-article:10477697 | pubmed:meshHeading | pubmed-meshheading:10477697... | lld:pubmed |
| pubmed-article:10477697 | pubmed:meshHeading | pubmed-meshheading:10477697... | lld:pubmed |
| pubmed-article:10477697 | pubmed:meshHeading | pubmed-meshheading:10477697... | lld:pubmed |
| pubmed-article:10477697 | pubmed:meshHeading | pubmed-meshheading:10477697... | lld:pubmed |
| pubmed-article:10477697 | pubmed:meshHeading | pubmed-meshheading:10477697... | lld:pubmed |
| pubmed-article:10477697 | pubmed:meshHeading | pubmed-meshheading:10477697... | lld:pubmed |
| pubmed-article:10477697 | pubmed:meshHeading | pubmed-meshheading:10477697... | lld:pubmed |
| pubmed-article:10477697 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10477697 | pubmed:articleTitle | Suppression of interleukin-12 production by human monocytes after preincubation with lipopolysaccharide. | lld:pubmed |
| pubmed-article:10477697 | pubmed:affiliation | Hannover Medical University, Department of Dermatology and Allergology, Hannover, Germany. | lld:pubmed |
| pubmed-article:10477697 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10477697 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10477697 | lld:pubmed |
