| pubmed-article:10456332 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10456332 | lifeskim:mentions | umls-concept:C0006675 | lld:lifeskim |
| pubmed-article:10456332 | lifeskim:mentions | umls-concept:C0006772 | lld:lifeskim |
| pubmed-article:10456332 | lifeskim:mentions | umls-concept:C0021547 | lld:lifeskim |
| pubmed-article:10456332 | lifeskim:mentions | umls-concept:C0030685 | lld:lifeskim |
| pubmed-article:10456332 | lifeskim:mentions | umls-concept:C0680255 | lld:lifeskim |
| pubmed-article:10456332 | lifeskim:mentions | umls-concept:C0391871 | lld:lifeskim |
| pubmed-article:10456332 | lifeskim:mentions | umls-concept:C1283071 | lld:lifeskim |
| pubmed-article:10456332 | lifeskim:mentions | umls-concept:C1963578 | lld:lifeskim |
| pubmed-article:10456332 | lifeskim:mentions | umls-concept:C1998793 | lld:lifeskim |
| pubmed-article:10456332 | lifeskim:mentions | umls-concept:C1617580 | lld:lifeskim |
| pubmed-article:10456332 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:10456332 | pubmed:dateCreated | 1999-8-30 | lld:pubmed |
| pubmed-article:10456332 | pubmed:abstractText | Our previous studies have demonstrated that calmodulin binds to IP3R type I (IP3R1) in a Ca2+ dependent manner, which suggests that calmodulin regulates the IP3R1 channel. In the present study, we investigated real-time kinetics of interactions between calmodulin and IP3R1 as well as effects of calmodulin on IP3-induced Ca2+ release by purified and reconstituted IP3R1. Kinetic analysis revealed that calmodulin binds to IP3R1 in a Ca2+ dependent manner and that both association and dissociation phase consist of two components with time constants of k(a) = 4.46 x 10(2) and > 10(4) M(-1) s(-1) k(d) = 1.44 x 10(-2) and 1.17 x 10(-1) s(-1). The apparent dissociation constant was calculated to be 27.3 microM. The IP3-induced Ca2+ release through the purified and reconstituted IP3R1 was inhibited by Ca2+/calmodulin, in a dose dependent manner. We interpret our findings to mean that calmodulin binds to IP3R1 in a Ca2+ dependent manner to exert inhibitory effect on IP3R channel activity. This event may be one of the mechanisms governing the negative feedback regulation of IP3-induced Ca2+ release by Ca2+. | lld:pubmed |
| pubmed-article:10456332 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10456332 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10456332 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10456332 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10456332 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10456332 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10456332 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10456332 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10456332 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10456332 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:10456332 | pubmed:issn | 0014-5793 | lld:pubmed |
| pubmed-article:10456332 | pubmed:author | pubmed-author:MikoshibaKK | lld:pubmed |
| pubmed-article:10456332 | pubmed:author | pubmed-author:NatsumeTT | lld:pubmed |
| pubmed-article:10456332 | pubmed:author | pubmed-author:HirotaJJ | lld:pubmed |
| pubmed-article:10456332 | pubmed:author | pubmed-author:MichikawaTT | lld:pubmed |
| pubmed-article:10456332 | pubmed:author | pubmed-author:FuruichiTT | lld:pubmed |
| pubmed-article:10456332 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10456332 | pubmed:day | 6 | lld:pubmed |
| pubmed-article:10456332 | pubmed:volume | 456 | lld:pubmed |
| pubmed-article:10456332 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10456332 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10456332 | pubmed:pagination | 322-6 | lld:pubmed |
| pubmed-article:10456332 | pubmed:dateRevised | 2007-7-18 | lld:pubmed |
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| pubmed-article:10456332 | pubmed:meshHeading | pubmed-meshheading:10456332... | lld:pubmed |
| pubmed-article:10456332 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10456332 | pubmed:articleTitle | Calmodulin inhibits inositol 1,4,5-trisphosphate-induced calcium release through the purified and reconstituted inositol 1,4,5-trisphosphate receptor type 1. | lld:pubmed |
| pubmed-article:10456332 | pubmed:affiliation | Mikoshiba Calciosignal Net Project, Exploratory Research for Advanced Technology, Japan Science and Technology Corporation, Tokyo, Japan. jhirota@ims.u-tokyo.ac.jp | lld:pubmed |
| pubmed-article:10456332 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10456332 | pubmed:publicationType | In Vitro | lld:pubmed |
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