| pubmed-article:10447683 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10447683 | lifeskim:mentions | umls-concept:C0016030 | lld:lifeskim |
| pubmed-article:10447683 | lifeskim:mentions | umls-concept:C0007745 | lld:lifeskim |
| pubmed-article:10447683 | lifeskim:mentions | umls-concept:C1959616 | lld:lifeskim |
| pubmed-article:10447683 | lifeskim:mentions | umls-concept:C0021467 | lld:lifeskim |
| pubmed-article:10447683 | lifeskim:mentions | umls-concept:C1156439 | lld:lifeskim |
| pubmed-article:10447683 | lifeskim:mentions | umls-concept:C0021469 | lld:lifeskim |
| pubmed-article:10447683 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:10447683 | pubmed:dateCreated | 1999-9-21 | lld:pubmed |
| pubmed-article:10447683 | pubmed:abstractText | Phospholipids and sphingolipids are important precursors of lipid-derived second messengers such as diacylglycerol and ceramide, which participate in several signal transduction pathways and in that way mediate the effects of various agonists. The cross-talk between glycerophospholipid and sphingolipid metabolism was investigated by examining the effects of cell-permeable ceramides on phosphatidylcholine (PtdCho) and phosphatidylethanolamine (PtdEtn) synthesis in Rat-2 fibroblasts. Addition of short-chain C6-ceramide to the cells resulted in a dose- and time-dependent inhibition of the CDP-pathways for PtdCho and PtdEtn synthesis. Treatment of cells for 4 h with 50 microM C6-ceramide caused an 83% and a 56% decrease in incorporation of radiolabelled choline and ethanolamine into PtdCho and PtdEtn, respectively. Exposure of the cells for longer time-periods (>/= 16 h) to 50 microM C6-ceramide resulted in apoptosis. The structural analogue dihydro-C6-ceramide did not affect PtdCho and PtdEtn synthesis. In pulse-chase experiments, radioactive choline and ethanolamine accumulated in CDP-choline and CDP-ethanolamine under the influence of C6-ceramide, suggesting that synthesis of both PtdCho and PtdEtn were inhibited at the final step in the CDP-pathways. Indeed, cholinephosphotransferase and ethanolaminephosphotransferase activities in membrane fractions from C6-ceramide-treated cells were reduced by 64% and 43%, respectively, when compared with control cells. No changes in diacylglycerol mass levels or synthesis of diacylglycerol from radiolabelled palmitate were observed. It was concluded that C6-ceramide affected glycerophospholipid synthesis predominantly by inhibition of the step in the CDP-pathways catalysed by cholinephosphotransferase and ethanolaminephosphotransferase. | lld:pubmed |
| pubmed-article:10447683 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10447683 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10447683 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10447683 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10447683 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10447683 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10447683 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10447683 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10447683 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10447683 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10447683 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10447683 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:10447683 | pubmed:issn | 0014-2956 | lld:pubmed |
| pubmed-article:10447683 | pubmed:author | pubmed-author:KleinWW | lld:pubmed |
| pubmed-article:10447683 | pubmed:author | pubmed-author:GeelenM JMJ | lld:pubmed |
| pubmed-article:10447683 | pubmed:author | pubmed-author:Van GoldeL... | lld:pubmed |
| pubmed-article:10447683 | pubmed:author | pubmed-author:HouwelingMM | lld:pubmed |
| pubmed-article:10447683 | pubmed:author | pubmed-author:BussièreMM | lld:pubmed |
| pubmed-article:10447683 | pubmed:author | pubmed-author:BladergroenB... | lld:pubmed |
| pubmed-article:10447683 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10447683 | pubmed:volume | 264 | lld:pubmed |
| pubmed-article:10447683 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10447683 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10447683 | pubmed:pagination | 152-60 | lld:pubmed |
| pubmed-article:10447683 | pubmed:dateRevised | 2007-7-23 | lld:pubmed |
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| pubmed-article:10447683 | pubmed:meshHeading | pubmed-meshheading:10447683... | lld:pubmed |
| pubmed-article:10447683 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10447683 | pubmed:articleTitle | Inhibition of phosphatidylcholine and phosphatidylethanolamine biosynthesis in rat-2 fibroblasts by cell-permeable ceramides. | lld:pubmed |
| pubmed-article:10447683 | pubmed:affiliation | Department of Biochemistry and Cell Biology, Graduate School of Animal Health, Utrecht University, The Netherlands. | lld:pubmed |
| pubmed-article:10447683 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10447683 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10447683 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10447683 | lld:pubmed |
