| pubmed-article:10438539 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10438539 | lifeskim:mentions | umls-concept:C0012155 | lld:lifeskim |
| pubmed-article:10438539 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:10438539 | lifeskim:mentions | umls-concept:C0038323 | lld:lifeskim |
| pubmed-article:10438539 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:10438539 | lifeskim:mentions | umls-concept:C0034987 | lld:lifeskim |
| pubmed-article:10438539 | lifeskim:mentions | umls-concept:C1167622 | lld:lifeskim |
| pubmed-article:10438539 | lifeskim:mentions | umls-concept:C0032615 | lld:lifeskim |
| pubmed-article:10438539 | lifeskim:mentions | umls-concept:C2362518 | lld:lifeskim |
| pubmed-article:10438539 | lifeskim:mentions | umls-concept:C1707511 | lld:lifeskim |
| pubmed-article:10438539 | lifeskim:mentions | umls-concept:C0441712 | lld:lifeskim |
| pubmed-article:10438539 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:10438539 | lifeskim:mentions | umls-concept:C0301625 | lld:lifeskim |
| pubmed-article:10438539 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:10438539 | pubmed:issue | 33 | lld:pubmed |
| pubmed-article:10438539 | pubmed:dateCreated | 1999-9-1 | lld:pubmed |
| pubmed-article:10438539 | pubmed:abstractText | Polyunsaturated fatty acids (PUFA) coordinately suppress the transcription of a wide array of hepatic lipogenic genes including fatty acid synthase (FAS) and acetyl-CoA carboxylase. Interestingly, the over-expression of sterol regulatory element binding protein-1 (SREBP-1) induces the expression of all of the enzymes suppressed by PUFA. This observation led us to hypothesize that PUFA coordinately inhibit lipogenic gene transcription by suppressing the expression of SREBP-1. Our initial studies revealed that the SREBP-1 and FAS mRNA contents of HepG2 cells were reduced by 20:4(n-6) in a dose-dependent manner (i.e. EC(50) approximately 10 microM), whereas 18:1(n-9) had no effect. Similarly, supplementing a fat-free, high glucose diet with oils rich in (n-6) or (n-3) PUFA reduced the hepatic content of precursor and nuclear SREBP-1 60 and 85%, respectively; however, PUFA had no effect on the nuclear content of upstream stimulatory factor (USF)-1. The PUFA-dependent decrease in nuclear content of mature SREBP-1 was paralleled by a 70-90% suppression in FAS gene transcription. In contrast, dietary 18:1(n-9), i.e. triolein, had no inhibitory influence on the expression of SREBP-1 or FAS. The decrease in hepatic expression of SREBP-1 and FAS associated with PUFA ingestion was mimicked by supplementing the fat-free diet with the PPARalpha-activator, WY 14, 643. Interestingly, nuclear run-on assays revealed that changes in SREBP-1 mRNA abundance were not accompanied by changes in SREBP-1 gene transcription. These results support the concept that PUFA coordinately inhibit lipogenic gene transcription by suppressing the expression of SREBP-1 and that the PUFA regulation of SREBP-1 appears to occur at the post-transcriptional level. | lld:pubmed |
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| pubmed-article:10438539 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:10438539 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:10438539 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10438539 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:10438539 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:10438539 | pubmed:author | pubmed-author:ClarkeS DSD | lld:pubmed |
| pubmed-article:10438539 | pubmed:author | pubmed-author:LinP SPS | lld:pubmed |
| pubmed-article:10438539 | pubmed:author | pubmed-author:XuJJ | lld:pubmed |
| pubmed-article:10438539 | pubmed:author | pubmed-author:NakamuraM TMT | lld:pubmed |
| pubmed-article:10438539 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10438539 | pubmed:day | 13 | lld:pubmed |
| pubmed-article:10438539 | pubmed:volume | 274 | lld:pubmed |
| pubmed-article:10438539 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10438539 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10438539 | pubmed:pagination | 23577-83 | lld:pubmed |
| pubmed-article:10438539 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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| pubmed-article:10438539 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10438539 | pubmed:articleTitle | Sterol regulatory element binding protein-1 expression is suppressed by dietary polyunsaturated fatty acids. A mechanism for the coordinate suppression of lipogenic genes by polyunsaturated fats. | lld:pubmed |
| pubmed-article:10438539 | pubmed:affiliation | Division of Nutritional Sciences and the Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, Texas 78712, USA. | lld:pubmed |
| pubmed-article:10438539 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10438539 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:10438539 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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