| pubmed-article:10404062 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10404062 | lifeskim:mentions | umls-concept:C0009402 | lld:lifeskim |
| pubmed-article:10404062 | lifeskim:mentions | umls-concept:C0105770 | lld:lifeskim |
| pubmed-article:10404062 | lifeskim:mentions | umls-concept:C1522484 | lld:lifeskim |
| pubmed-article:10404062 | lifeskim:mentions | umls-concept:C0036525 | lld:lifeskim |
| pubmed-article:10404062 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:10404062 | lifeskim:mentions | umls-concept:C0205225 | lld:lifeskim |
| pubmed-article:10404062 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:10404062 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:10404062 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:10404062 | pubmed:dateCreated | 1999-8-5 | lld:pubmed |
| pubmed-article:10404062 | pubmed:abstractText | beta-catenin plays a fundamental role in the regulation of the E-cadherin-catenin cell adhesion complex. It also functions in growth signalling events, independently of the cadherin-catenin complex, and these signalling pathways are disturbed in colorectal cancer. Mutations in either the APC or beta-catenin genes in colorectal cancer cells result in up-regulation of protein expression and subsequent cytoplasmic and nuclear distribution of beta-catenin. In this study, we examined beta-catenin expression in 47 primary colorectal tumors and the corresponding liver metastases. Immunohistochemical studies demonstrated loss of membranous beta-catenin expression in 26% of primary tumors and 60% of liver metastases and a concomitant increase in cytoplasmic and nuclear staining. Widespread nuclear expression of beta-catenin was found in 64% of primary tumors and 21% of liver metastases. No associations were found between any form of beta-catenin expression and either tumor stage or tumor grade. Cellular distribution of beta-catenin was also examined by detergent extraction and Western blot analysis in 16 primary tumors and 23 liver metastases. This analysis showed that most tumors demonstrated reduced beta-catenin in the cytoskeletal fraction and increased beta-catenin in the cytosolic fraction. Furthermore, 3 liver metastases were found to contain a truncated beta-catenin protein of approximately M(r) 80,000. Immunoprecipitation studies showed that the truncated beta-catenin proteins only bound weakly to E-cadherin and beta-catenin compared with non-truncated beta-catenin. These results demonstrate gross alterations in the cellular distribution of beta-catenin in primary colorectal cancers with metastatic potential, as well as in the metastatic tumors. These changes may be the consequence of APC or beta-catenin gene mutations, or possibly result from a post-translational modification of the E-cadherin-catenin complex. | lld:pubmed |
| pubmed-article:10404062 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10404062 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10404062 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10404062 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10404062 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10404062 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10404062 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10404062 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10404062 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10404062 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:10404062 | pubmed:issn | 0020-7136 | lld:pubmed |
| pubmed-article:10404062 | pubmed:author | pubmed-author:KinsellaA RAR | lld:pubmed |
| pubmed-article:10404062 | pubmed:author | pubmed-author:PostonG JGJ | lld:pubmed |
| pubmed-article:10404062 | pubmed:author | pubmed-author:HughT JTJ | lld:pubmed |
| pubmed-article:10404062 | pubmed:author | pubmed-author:PignatelliMM | lld:pubmed |
| pubmed-article:10404062 | pubmed:author | pubmed-author:GettyBB | lld:pubmed |
| pubmed-article:10404062 | pubmed:author | pubmed-author:O'DowdGG | lld:pubmed |
| pubmed-article:10404062 | pubmed:author | pubmed-author:DillonS ASA | lld:pubmed |
| pubmed-article:10404062 | pubmed:copyrightInfo | Copyright 1999 Wiley-Liss, Inc. | lld:pubmed |
| pubmed-article:10404062 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10404062 | pubmed:day | 12 | lld:pubmed |
| pubmed-article:10404062 | pubmed:volume | 82 | lld:pubmed |
| pubmed-article:10404062 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10404062 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10404062 | pubmed:pagination | 504-11 | lld:pubmed |
| pubmed-article:10404062 | pubmed:dateRevised | 2007-7-24 | lld:pubmed |
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| pubmed-article:10404062 | pubmed:meshHeading | pubmed-meshheading:10404062... | lld:pubmed |
| pubmed-article:10404062 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10404062 | pubmed:articleTitle | beta-catenin expression in primary and metastatic colorectal carcinoma. | lld:pubmed |
| pubmed-article:10404062 | pubmed:affiliation | Cellular Oncology Group, Department of Surgery, University of Liverpool, Liverpool, UK. thugh@med.usyd.edu.au | lld:pubmed |
| pubmed-article:10404062 | pubmed:publicationType | Journal Article | lld:pubmed |
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