pubmed-article:10400718 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:10400718 | lifeskim:mentions | umls-concept:C0019704 | lld:lifeskim |
pubmed-article:10400718 | lifeskim:mentions | umls-concept:C0205419 | lld:lifeskim |
pubmed-article:10400718 | lifeskim:mentions | umls-concept:C0019409 | lld:lifeskim |
pubmed-article:10400718 | lifeskim:mentions | umls-concept:C1519249 | lld:lifeskim |
pubmed-article:10400718 | lifeskim:mentions | umls-concept:C0370215 | lld:lifeskim |
pubmed-article:10400718 | lifeskim:mentions | umls-concept:C0449560 | lld:lifeskim |
pubmed-article:10400718 | lifeskim:mentions | umls-concept:C0024548 | lld:lifeskim |
pubmed-article:10400718 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
pubmed-article:10400718 | pubmed:issue | 8 | lld:pubmed |
pubmed-article:10400718 | pubmed:dateCreated | 1999-8-24 | lld:pubmed |
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pubmed-article:10400718 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
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pubmed-article:10400718 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:10400718 | pubmed:abstractText | We have examined the nature of V3 sequence variability among subtype C human immunodeficiency virus type 1 (HIV-1) sequences from plasma-derived viral RNA present in infected men from Malawi. Sequence variability was assessed by direct sequence analysis of the V3 reverse transcription-PCR products, examination of virus populations by a subtype C V3-specific heteroduplex tracking assay (V3-HTA), and selected sequence analysis of molecular clones derived from the PCR products. Sequence variability in V3 among the subtype C viruses was not associated with the presence of basic amino acid substitutions. This observation is in contrast to that for subtype B HIV-1, where sequence variability is associated with such substitutions, and these substitutions are determinants of altered coreceptor usage. Evolutionary variants in subtype C V3 sequences, as defined by the V3-HTA, were not correlated with the CD4 level in the infected person, while such a correlation was found with subtype B V3 sequences. Viruses were isolated from a subset of the subjects; all isolates used CCR5 and not CXCR4 as a coreceptor, and none was able to grow in MT-2 cells, a hallmark of the syncytium-inducing phenotype that is correlated with CXCR4 usage. The overall sequence variability of the subtype C V3 region was no greater than that of the conserved regions of gp120. This limited sequence variability was also a feature of subtype B V3 sequences that do not carry the basic amino acid substitutions associated with altered coreceptor usage. Our results indicate that altered coreceptor usage is rare in subtype C HIV-1 isolates in sub-Saharan Africa and that sequence variability is not a feature of the V3 region of env in the absence of altered coreceptor usage. | lld:pubmed |
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