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| pubmed-article:10388555 | lifeskim:mentions | umls-concept:C0019682 | lld:lifeskim |
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| pubmed-article:10388555 | lifeskim:mentions | umls-concept:C0024432 | lld:lifeskim |
| pubmed-article:10388555 | lifeskim:mentions | umls-concept:C0441471 | lld:lifeskim |
| pubmed-article:10388555 | lifeskim:mentions | umls-concept:C1704632 | lld:lifeskim |
| pubmed-article:10388555 | lifeskim:mentions | umls-concept:C0871261 | lld:lifeskim |
| pubmed-article:10388555 | lifeskim:mentions | umls-concept:C2911692 | lld:lifeskim |
| pubmed-article:10388555 | lifeskim:mentions | umls-concept:C1706817 | lld:lifeskim |
| pubmed-article:10388555 | lifeskim:mentions | umls-concept:C2611831 | lld:lifeskim |
| pubmed-article:10388555 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:10388555 | lifeskim:mentions | umls-concept:C1546857 | lld:lifeskim |
| pubmed-article:10388555 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:10388555 | pubmed:dateCreated | 1999-8-3 | lld:pubmed |
| pubmed-article:10388555 | pubmed:abstractText | Human immunodeficiency virus type 1 (HIV-1) Nef is important for viral infectivity and pathogenicity. HIV-1 infection is associated with inappropriate activation and defects in the function of monocytes/macrophages. We have studied the effects of HIV-1 Nef in the murine (RAW264.7) and human (THP-1) monocyte-macrophage cell lines. Investigation of the activator protein-1 (AP-1) transcription factor showed that Nef expression induced both its DNA binding and transcriptional activities. Increased AP-1 DNA binding activity in RAW264.7 cells was associated with raised levels of c-Fos expression and induction of mRNA for the AP-1 responsive tissue inhibitor of metalloproteinases-1 (TIMP-1) gene. Mutagenesis and kinase inhibition studies were employed to determine signaling pathways used by Nef to induce AP-1. Data from these studies indicated that induction of AP-1 by Nef is likely to be mediated through the MAPK (ERK1 and 2) signaling pathway and requires the proline-rich PxxP motif of Nef, suggesting the involvement of upstream protein kinases belonging to the Src family. Effects of Nef on AP-1 induction were cell lineage-specific, being stimulatory in macrophages, inhibitory in T cells and without effect in HeLa cells. These latter two observations led us to test the possibility that cell-specific interactions of Nef with Src family proteins may modulate AP-1 activity. To this end we demonstrated that a dominant-negative Hck mutant caused inhibition of Nef-mediated AP-1 DNA binding activity in RAW cells. In conclusion, induction of AP-1 by Nef is a specific feature of human and murine macrophage cell lines that requires signal transduction events involving Hck and MAPKs. | lld:pubmed |
| pubmed-article:10388555 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10388555 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10388555 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:10388555 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10388555 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:10388555 | pubmed:issn | 0022-2836 | lld:pubmed |
| pubmed-article:10388555 | pubmed:author | pubmed-author:BartonC HCH | lld:pubmed |
| pubmed-article:10388555 | pubmed:author | pubmed-author:CraggRR | lld:pubmed |
| pubmed-article:10388555 | pubmed:author | pubmed-author:MannD ADA | lld:pubmed |
| pubmed-article:10388555 | pubmed:author | pubmed-author:SakselaKK | lld:pubmed |
| pubmed-article:10388555 | pubmed:author | pubmed-author:HarrisM PMP | lld:pubmed |
| pubmed-article:10388555 | pubmed:author | pubmed-author:BiggsT ETE | lld:pubmed |
| pubmed-article:10388555 | pubmed:copyrightInfo | Copyright 1999 Academic Press. | lld:pubmed |
| pubmed-article:10388555 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10388555 | pubmed:day | 2 | lld:pubmed |
| pubmed-article:10388555 | pubmed:volume | 290 | lld:pubmed |
| pubmed-article:10388555 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10388555 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10388555 | pubmed:pagination | 21-35 | lld:pubmed |
| pubmed-article:10388555 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
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| pubmed-article:10388555 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10388555 | pubmed:articleTitle | Induction of activator protein 1 (AP-1) in macrophages by human immunodeficiency virus type-1 NEF is a cell-type-specific response that requires both hck and MAPK signaling events. | lld:pubmed |
| pubmed-article:10388555 | pubmed:affiliation | Southampton General Hospital, Southampton, SO16 6YD, UK. | lld:pubmed |
| pubmed-article:10388555 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10388555 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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