| pubmed-article:10360813 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10360813 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:10360813 | lifeskim:mentions | umls-concept:C0085295 | lld:lifeskim |
| pubmed-article:10360813 | lifeskim:mentions | umls-concept:C0017636 | lld:lifeskim |
| pubmed-article:10360813 | lifeskim:mentions | umls-concept:C0024432 | lld:lifeskim |
| pubmed-article:10360813 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:10360813 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:10360813 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:10360813 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:10360813 | pubmed:dateCreated | 1999-6-17 | lld:pubmed |
| pubmed-article:10360813 | pubmed:abstractText | Interleukin 10 (IL-10) expression has been found to be correlated with the extent of malignancy in gliomas. In vitro, IL-10 increases proliferation and migratory capacity in human glioma cell lines. In this study, we localized the site of IL-10 synthesis in gliomas to cells of microglial origin. Biopsy specimens from 11 patients with malignant glioma were processed on native tissues and at early cell culture passages (0-4). IL-10 mRNA was analyzed by RT-PCR and in situ hybridization. Protein was quantitatively assessed by ELISA in cell culture supernatants, and cells expressing IL-10 were determined by a combination of immunohistochemistry for CD68 (specific for microglia/macrophage lineage) and IL-10 in situ hybridization. IL-10 mRNA decreased from passage 0 to 4 in all samples and was undetectable beyond passage 5. Such downregulation of mRNA leads to a steep decrease of IL-10 protein in culture supernatants (below detection level, 0.05 ng/ml, beyond passage 1). The combination of in situ hybridization for IL-10 and CD68 immunostaining revealed that only cells of the microglia/macrophage lineage produced IL-10 mRNA. Our results identify microglia/macrophage cells as the major source of IL-10 expression in gliomas which decreases markedly during early passages of primary cultures of human gliomas due to a progressive reduction of microglia/macrophages present. | lld:pubmed |
| pubmed-article:10360813 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10360813 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10360813 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10360813 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10360813 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10360813 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10360813 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10360813 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10360813 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10360813 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:10360813 | pubmed:issn | 0020-7136 | lld:pubmed |
| pubmed-article:10360813 | pubmed:author | pubmed-author:RoosenKK | lld:pubmed |
| pubmed-article:10360813 | pubmed:author | pubmed-author:RoggendorfWW | lld:pubmed |
| pubmed-article:10360813 | pubmed:author | pubmed-author:WagnerSS | lld:pubmed |
| pubmed-article:10360813 | pubmed:author | pubmed-author:GreibII | lld:pubmed |
| pubmed-article:10360813 | pubmed:author | pubmed-author:RieckmannPP | lld:pubmed |
| pubmed-article:10360813 | pubmed:author | pubmed-author:TonnJ CJC | lld:pubmed |
| pubmed-article:10360813 | pubmed:author | pubmed-author:KerkarPP | lld:pubmed |
| pubmed-article:10360813 | pubmed:author | pubmed-author:CzubSS | lld:pubmed |
| pubmed-article:10360813 | pubmed:author | pubmed-author:VinceG HGH | lld:pubmed |
| pubmed-article:10360813 | pubmed:author | pubmed-author:SüthZZ | lld:pubmed |
| pubmed-article:10360813 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10360813 | pubmed:day | 2 | lld:pubmed |
| pubmed-article:10360813 | pubmed:volume | 82 | lld:pubmed |
| pubmed-article:10360813 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10360813 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10360813 | pubmed:pagination | 12-6 | lld:pubmed |
| pubmed-article:10360813 | pubmed:dateRevised | 2007-7-24 | lld:pubmed |
| pubmed-article:10360813 | pubmed:meshHeading | pubmed-meshheading:10360813... | lld:pubmed |
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| pubmed-article:10360813 | pubmed:meshHeading | pubmed-meshheading:10360813... | lld:pubmed |
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| pubmed-article:10360813 | pubmed:meshHeading | pubmed-meshheading:10360813... | lld:pubmed |
| pubmed-article:10360813 | pubmed:meshHeading | pubmed-meshheading:10360813... | lld:pubmed |
| pubmed-article:10360813 | pubmed:meshHeading | pubmed-meshheading:10360813... | lld:pubmed |
| pubmed-article:10360813 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10360813 | pubmed:articleTitle | Microglial/macrophage expression of interleukin 10 in human glioblastomas. | lld:pubmed |
| pubmed-article:10360813 | pubmed:affiliation | Department of Neurosurgery, University of Würzburg, Germany. | lld:pubmed |
| pubmed-article:10360813 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10360813 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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