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pubmed-article:10358742pubmed:abstractTextWe have cloned and characterised a cDNA encoding Z protein of recently identified MX strain of lymphocytic choriomeningitis virus (LCMV) persistently infecting human MaTu cells. Deduced amino acid sequence of LCMV MX Z protein showed 88.9% identity with that of the LCMV Armstrong (ARM) strain and 80.9% identity with that of the LCMV Traub (TRA) strain. It contained conserved zinc-binding RING finger domain and C-terminal proline-rich region. Northern blot analysis of total RNA from MaTu cells revealed presence of abundant truncated forms of L RNA. Z protein-specific rabbit antibodies were produced to glutathione S-transferase (GST)-Z fusion protein expressed in E. coli and used for the detection of Z protein in MaTu cells. Western blot and immunofluorescence analyses detected relatively high levels of Z protein indicating its role in maintenance of persistent LCMV.lld:pubmed
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pubmed-article:10358742pubmed:articleTitleSequence and characterisation of the Z gene encoding ring finger protein of the lymphocytic choriomeningitis virus MX strain.lld:pubmed
pubmed-article:10358742pubmed:affiliationInstitute of Virology, Slovak Academy of Sciences, Bratislava, Slovak Republic.lld:pubmed
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pubmed-article:10358742pubmed:publicationTypeComparative Studylld:pubmed
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