Linked Life Data

10348865

Source:http://linkedlifedata.com/resource/pubmed/id/10348865

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pubmed-article:10348865pubmed:abstractTextWe generated random mutations in Synechococcus sp. strain PCC 7942 to look for genes of output pathways in the cyanobacterial circadian system. A derivative of transposon Tn5 was introduced into the chromosomes of reporter strains in which cyanobacterial promoters drive the Vibrio harveyi luxAB genes and produce an oscillation of bioluminescence as a function of circadian gene expression. Among low-amplitude mutants, one mutant, tnp6, had an insertion in a 780-bp open reading frame. The tnp6 mutation produced an altered circadian phasing phenotype in the expression rhythms of psbAI::luxAB, psbAII::luxAB, and kaiA::luxAB but had no or little effect on those of psbAIII::luxAB, purF::luxAB, kaiB::luxAB, rpoD2::luxAB, ndhD::luxAB, and conII::luxAB. This suggests that the interrupted gene in tnp6, named cpmA (circadian phase modifier), is part of a circadian output pathway that regulates the expression rhythms of psbAI, psbAII, and kaiA.lld:pubmed
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pubmed-article:10348865pubmed:articleTitlecpmA, a gene involved in an output pathway of the cyanobacterial circadian system.lld:pubmed
pubmed-article:10348865pubmed:affiliationDepartment of Biology, Texas A&M University, College Station, Texas 77843-3258, USA.lld:pubmed
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