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pubmed-article:10347767pubmed:abstractTextWe have developed a polymerase chain reaction (PCR) assay which distinguishes genotype F from the other genotypes of hepatitis B virus (HBV). The method was used to characterize HBV strains isolated in urban areas of the Brazilian Amazon. DNA was amplified in 54 of a total of 78 HBsAg-positive serum samples, using universal, non-genotype-specific primers. Only 4 (7.4%) were identified as genotype F by our genotype-specific PCR assay. This proportion is notably lower than that previously reported in Argentina, Venezuela, Peru, and Central America.lld:pubmed
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pubmed-article:10347767pubmed:authorpubmed-author:MorganT FTFlld:pubmed
pubmed-article:10347767pubmed:authorpubmed-author:NielCClld:pubmed
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pubmed-article:10347767pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:10347767pubmed:year1999lld:pubmed
pubmed-article:10347767pubmed:articleTitleA polymerase chain reaction-based assay to identify genotype F of hepatitis B virus.lld:pubmed
pubmed-article:10347767pubmed:affiliationDepartamento de Virologia, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, Brasil.lld:pubmed
pubmed-article:10347767pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:10347767pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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