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pubmed-article:1028376pubmed:abstractTextAdult rainbow trout were immunized intraperitoneally with a chloroform killed Aeromonas salmonicida suspension. Agglutinating antibodies were titrated using a microtiter system. Antibody activity was associated with IgM fraction obtained from Sepharose 4 B gel filtration. The kinetics of antibody formation were established at three temperatures. At 50C detectable antibodies were found at day 50 post immunization. At 10degrees C and 16 degrees C the agglutinin titer rose before day 20. The antibody titer was significantly raised by Freund's complete adjuvant, but cyclophosphamide at a total dose of 500 mg/kg of body weight administered in 10 injections before and after immunization had no effect.lld:pubmed
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pubmed-article:1028376pubmed:pagination307-13lld:pubmed
pubmed-article:1028376pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:1028376pubmed:year1976lld:pubmed
pubmed-article:1028376pubmed:articleTitle[Production of anti-Aeromonas salmonicida agglutinins by the rainbow trout. Influence of temperature, an adjuvant and an immunosuppressive agent].lld:pubmed
pubmed-article:1028376pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:1028376pubmed:publicationTypeEnglish Abstractlld:pubmed