Linked Life Data

10235125

Source:http://linkedlifedata.com/resource/pubmed/id/10235125

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pubmed-article:10235125pubmed:abstractTextThe pathogenesis of Pneumocystis carinii pneumonia (PCP) suggests an important role for dysfunction of the pulmonary surfactant system in the hypoxemic respiratory insufficiency associated with this infection. Surfactant protein B (SP-B) is a hydrophobic protein shown to be essential for normal surfactant function in vivo. Therefore, we hypothesized that the inhibition of SP-B expression occurs during PCP, and we tested this hypothesis in two immunodeficient animal models. PCP was induced in C.B-17 scid/scid mice by intratracheal inoculation of P. carinii organisms. Infected lung homogenates, obtained at time points up to 6 weeks after inoculation, were analyzed for SP-B and mRNA content. When a comparison was made with uninfected scid controls, the densitometric quantitation of Western blots of lung homogenates demonstrated significant reductions in 8 kd SP-B in mice infected with P. carinii 4 weeks after inoculation (16% of the control value). Northern blot analysis showed a concomitant decrease in SP-B mRNA to 24% of the control level. The decrease in SP-B and mRNA levels in lung homogenates of infected mice was reflected in lower SP-B levels in the surfactant. An enzyme-linked immunosorbent assay for the SP-B level in surfactant prepared from bronchoalveolar lavage samples of infected scid mice demonstrated a significant reduction in alveolar SP-B content (45% of the control value). In contrast to the results with SP-B, neither the SP-A protein content nor the mRNA level was significantly altered by PCP infection. To confirm these observations, SP-B expression was studied in an additional animal model of PCP. The SP-B content of lung homogenates from BALB/c mice depleted of CD4+ T cells and infected with P. carinii was also reduced (51% of the control value). We conclude that P. carinii induces selective inhibition of the expression of SP-B in two mouse models of PCP and that this down-regulation is mediated at the level of mRNA expression. Therefore, an acquired deficiency of SP-B is likely to be an important contributor to the pathogenesis of hypoxemic respiratory failure that is observed in patients with PCP.lld:pubmed
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pubmed-article:10235125pubmed:authorpubmed-author:PrestonA MAMlld:pubmed
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pubmed-article:10235125pubmed:pagination423-33lld:pubmed
pubmed-article:10235125pubmed:dateRevised2007-11-14lld:pubmed
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pubmed-article:10235125pubmed:articleTitleInhibition of lung surfactant protein B expression during Pneumocystis carinii pneumonia in mice.lld:pubmed
pubmed-article:10235125pubmed:affiliationDepartment of Medicine, University of Pennsylvania School of Medicine, Philadelphia 19104-6068, USA.lld:pubmed
pubmed-article:10235125pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:10235125pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
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