| pubmed-article:10233691 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10233691 | lifeskim:mentions | umls-concept:C0021757 | lld:lifeskim |
| pubmed-article:10233691 | lifeskim:mentions | umls-concept:C0004827 | lld:lifeskim |
| pubmed-article:10233691 | lifeskim:mentions | umls-concept:C0079460 | lld:lifeskim |
| pubmed-article:10233691 | lifeskim:mentions | umls-concept:C0037473 | lld:lifeskim |
| pubmed-article:10233691 | lifeskim:mentions | umls-concept:C0013081 | lld:lifeskim |
| pubmed-article:10233691 | lifeskim:mentions | umls-concept:C0019595 | lld:lifeskim |
| pubmed-article:10233691 | lifeskim:mentions | umls-concept:C1704259 | lld:lifeskim |
| pubmed-article:10233691 | lifeskim:mentions | umls-concept:C1705987 | lld:lifeskim |
| pubmed-article:10233691 | lifeskim:mentions | umls-concept:C1524003 | lld:lifeskim |
| pubmed-article:10233691 | lifeskim:mentions | umls-concept:C0205214 | lld:lifeskim |
| pubmed-article:10233691 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:10233691 | pubmed:dateCreated | 1999-7-16 | lld:pubmed |
| pubmed-article:10233691 | pubmed:abstractText | Granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-3 (IL-3) are recognized as enhancers, but not as inducers, of histamine release from normal human basophils. However, when extracellular Na+ is removed IL-3 acquires the capacity to induce histamine release. The aim of this study was to evaluate whether GM-CSF can induce basophil histamine release using the same pathway of IL-3. Leucocyte suspensions from normal human subjects were stimulated with GM-CSF, IL-3 and anti-IgE, and histamine release was evaluated by an automated fluorometric method. In a physiological medium, GM-CSF (10 ng/ml) and IL-3 (10 ng/ml) did not provoke histamine release, in spite of an efficient response to anti-IgE (10 micrograms/ml). However, when extracellular Na+ was substituted iso-osmotically with N-methyl-d-glucamine+ or with choline+, GM-CSF and IL-3 were able to trigger histamine release from either mixed leucocyte suspensions or purified human basophils. The effect of GM-CSF on basophil histamine release was dose dependent, with optimal release at a dose of 1 ng/ml after incubation at 37 degrees for 60-120 min. The kinetics of IL-3-induced histamine release were similar, whereas anti-IgE-induced histamine release was more rapid, being almost maximal after incubation for 30 min. A good correlation was found between GM-CSF-induced and IL-3-induced histamine release; furthermore, the combined effects of the two cytokines were less than additive, suggesting that they share the same pathways leading to histamine release. When extracellular Na+ concentration was increased from 0 to 140 mm, histamine release induced by GM-CSF, IL-3 and anti-IgE was reduced progressively. In contrast, histamine release induced by these stimuli was upregulated when the concentration of extracellular Ca2+ was increased. These results provide indirect evidence that GM-CSF and IL-3 can induce basophil histamine release by a common pathway that is downregulated by Na+. | lld:pubmed |
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| pubmed-article:10233691 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10233691 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10233691 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10233691 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:10233691 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10233691 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:10233691 | pubmed:issn | 0019-2805 | lld:pubmed |
| pubmed-article:10233691 | pubmed:author | pubmed-author:Di DonatoMM | lld:pubmed |
| pubmed-article:10233691 | pubmed:author | pubmed-author:MiadonnaAA | lld:pubmed |
| pubmed-article:10233691 | pubmed:author | pubmed-author:TedeschiAA | lld:pubmed |
| pubmed-article:10233691 | pubmed:author | pubmed-author:LoriniMM | lld:pubmed |
| pubmed-article:10233691 | pubmed:author | pubmed-author:SalmasoCC | lld:pubmed |
| pubmed-article:10233691 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10233691 | pubmed:volume | 96 | lld:pubmed |
| pubmed-article:10233691 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10233691 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10233691 | pubmed:pagination | 164-70 | lld:pubmed |
| pubmed-article:10233691 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:10233691 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10233691 | pubmed:articleTitle | Granulocyte-macrophage colony-stimulating factor and interleukin-3 cause basophil histamine release by a common pathway: downregulation by sodium. | lld:pubmed |
| pubmed-article:10233691 | pubmed:affiliation | Respiratory Allergy and Immunology Unit, Third Division of Internal Medicine, IRCCS Ospedale Maggiore Policlinico, Milan, Italy. | lld:pubmed |
| pubmed-article:10233691 | pubmed:publicationType | Journal Article | lld:pubmed |
