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pubmed-article:10230033pubmed:abstractTextA micro-biosensor was constructed by incorporating the organic conducting salt tetrathiafulvalene-tetracyanoquinodimethane (TTF-TCNQ) into a platinized platinum (Pt) wire and further covering with the electrochemical polymerical heteropolypyrrole film, in which glucose oxidase (GOx) was entrapped. The enzyme electrode can sensitively determine glucose at a low working potential, mainly based on the oxidation of H2O2. The incorporated TTF-TCNQ can significantly improve the oxidation of H2O2 on the electrode, although a part of the TTF-TCNQ functions as a mediator. Compared with the same electrode prepared without TTF-TCNQ incorporated, the TTF-TCNQ modified electrode had better performance characteristics at a working potential of 200 mV (versus SCE). The response time to 90% of the steady value was shortened from about 40 s to less than 10 s, the lower limit of the linear response was greatly extended from about 1.6 mM to 10 microM, the linear range was shifted from 1.6-10.0 to 0.01-5 mM and the sensitivity was increased from about 1 to 1.5 microA/mM. The electrode was quite stable. For continuous operation, the electrode could work for about 5 weeks and only lost 60% of its original sensitivity. Stored at 4 degrees C for intermittent determinations, the electrode kept 80% sensitivity for over 6 months. Due to covering the electrode with a non-conductive heteropolypyrrole film, ascorbate, urate and 4-acetamidophenol caused only negligible current response at an applied potential of 200 mV.lld:pubmed
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pubmed-article:10230033pubmed:authorpubmed-author:YuB SBSlld:pubmed
pubmed-article:10230033pubmed:authorpubmed-author:LimB PBPlld:pubmed
pubmed-article:10230033pubmed:authorpubmed-author:LiQ SQSlld:pubmed
pubmed-article:10230033pubmed:authorpubmed-author:ZhongJ JJJlld:pubmed
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pubmed-article:10230033pubmed:pagination327-34lld:pubmed
pubmed-article:10230033pubmed:dateRevised2009-7-14lld:pubmed
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pubmed-article:10230033pubmed:year1999lld:pubmed
pubmed-article:10230033pubmed:articleTitleImprovement of the performance of H2O2 oxidation at low working potential by incorporating TTF-TCNQ into a platinum wire electrode for glucose determination.lld:pubmed
pubmed-article:10230033pubmed:affiliationState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, People's Republic of China.lld:pubmed
pubmed-article:10230033pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:10230033pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed