| pubmed-article:10222011 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10222011 | lifeskim:mentions | umls-concept:C0014442 | lld:lifeskim |
| pubmed-article:10222011 | lifeskim:mentions | umls-concept:C0015684 | lld:lifeskim |
| pubmed-article:10222011 | lifeskim:mentions | umls-concept:C0010762 | lld:lifeskim |
| pubmed-article:10222011 | lifeskim:mentions | umls-concept:C0596988 | lld:lifeskim |
| pubmed-article:10222011 | lifeskim:mentions | umls-concept:C0534137 | lld:lifeskim |
| pubmed-article:10222011 | lifeskim:mentions | umls-concept:C0549178 | lld:lifeskim |
| pubmed-article:10222011 | lifeskim:mentions | umls-concept:C1510438 | lld:lifeskim |
| pubmed-article:10222011 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:10222011 | pubmed:dateCreated | 1999-6-15 | lld:pubmed |
| pubmed-article:10222011 | pubmed:abstractText | Cytochrome P450 BM-3 from Bacillus megaterium catalyzes the subterminal hydroxylation of medium- and long-chain fatty acids at the positions omega-1, omega-2, and omega-3. A rapid and continuous spectrophotometric activity assay for cytochrome P450 BM-3 based on the conversion of p-nitrophenoxycarboxylic acids (pNCA) to omega-oxycarboxylic acids and the chromophore p-nitrophenolate was developed. In contrast to the commonly used activity assays for this enzyme, relying on the consumption of oxygen or NADPH or the use of 14C-labeled carboxylic acids, the pNCA assay can even be used with crude extracts of the recombinant enzyme from lysed Escherichia coli cells. The kinetics of p-nitrophenolate formation are directly measured at a wavelength of 410 nm using a spectrophotometer or microtiter plate reader. Sensitivity of the assay is greatly enhanced if p-nitrophenoxydodecanoic or p-nitrophenoxypentadecanoic acid are used with the F87A mutant instead of the wild-type P450 BM-3 enzyme. | lld:pubmed |
| pubmed-article:10222011 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10222011 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10222011 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10222011 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10222011 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:10222011 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10222011 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10222011 | pubmed:month | May | lld:pubmed |
| pubmed-article:10222011 | pubmed:issn | 0003-2697 | lld:pubmed |
| pubmed-article:10222011 | pubmed:author | pubmed-author:SchmittJJ | lld:pubmed |
| pubmed-article:10222011 | pubmed:author | pubmed-author:SchmidR DRD | lld:pubmed |
| pubmed-article:10222011 | pubmed:author | pubmed-author:Schmidt-Danne... | lld:pubmed |
| pubmed-article:10222011 | pubmed:author | pubmed-author:SchwanebergUU | lld:pubmed |
| pubmed-article:10222011 | pubmed:copyrightInfo | Copyright 1999 Academic Press. | lld:pubmed |
| pubmed-article:10222011 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10222011 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:10222011 | pubmed:volume | 269 | lld:pubmed |
| pubmed-article:10222011 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10222011 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10222011 | pubmed:pagination | 359-66 | lld:pubmed |
| pubmed-article:10222011 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
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| pubmed-article:10222011 | pubmed:meshHeading | pubmed-meshheading:10222011... | lld:pubmed |
| pubmed-article:10222011 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10222011 | pubmed:articleTitle | A continuous spectrophotometric assay for P450 BM-3, a fatty acid hydroxylating enzyme, and its mutant F87A. | lld:pubmed |
| pubmed-article:10222011 | pubmed:affiliation | Institut für Technische Biochemie, Universität Stuttgart, Allmandring 31, Stuttgart, 70569, Germany. | lld:pubmed |
| pubmed-article:10222011 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10222011 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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