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pubmed-article:10206757pubmed:abstractTextWe evaluated the in vitro activity of fluconazole, itraconazole, ketoconazole, 5-fluorocytosine and amphotericin B against 30 clinical isolates of Saccharomyces cerevisiae by a broth microdilution method, following the NCCLS recommendation. Testing was performed either in RPMI-1640 or yeast nitrogen base (YNB). YNB supported the growth of all isolates tested, while results in RPMI-1640 were not obtained for six isolates (20%). The MIC of all three azoles in YNB were one or two dilutions higher than those obtained in RPMI-1640 (P=0.0001 for fluconazole and itraconazole, P=0.03 for ketoconazole). Elevated MICs were observed for all three azoles, while all the isolates were susceptible to 5-fluorocytosine and amphotericin B. All MIC values were confirmed by spectrophotometric reading. Six strains of S. cerevisiae isolated from the faeces and consecutive blood cultures from an AIDS patient over a 7-month period were typed by electrophoretic karyotyping (EK). EK showed the maintenance of the same karyotype over time suggesting that the faecal isolate changed from a colonizing to infection-causing strain. The relative resistance of S. cerevisiae to azole drugs as well as its ability to cause widespread infections may promote the emergence of this species as a pathogen in immunosuppressed patients.lld:pubmed
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pubmed-article:10206757pubmed:dateRevised2009-11-19lld:pubmed
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pubmed-article:10206757pubmed:articleTitleIn vitro activity of five antifungal agents against clinical isolates of Saccharomyces cerevisiae.lld:pubmed
pubmed-article:10206757pubmed:affiliationInstitute of Infectious Diseases and Public Health of the University of Ancona, Italy. cmalinf@popcsi.unian.itlld:pubmed
pubmed-article:10206757pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:10206757pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed