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pubmed-article:10203037pubmed:abstractTextEnumeration of antigen-specific CD8+ T-cells has traditionally been largely dependent on the use of techniques that require a period of expansion in vitro. However the recent emergence of methods that can be used directly ex vivo to quantify antigen-specific CD8+ T cells has prompted a re-evaluation of the magnitude of viral-specific cellular immune responses. The purpose of this review is to describe the commonly used methods currently available to quantify and characterise antigen-specific T-cells and then to focus in more detail on the use of tetrameric complexes of HLA/peptide to study the cellular immune response.lld:pubmed
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pubmed-article:10203037pubmed:authorpubmed-author:McMichaelA...lld:pubmed
pubmed-article:10203037pubmed:authorpubmed-author:OggG SGSlld:pubmed
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pubmed-article:10203037pubmed:pagination77-80lld:pubmed
pubmed-article:10203037pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:10203037pubmed:articleTitleQuantitation of antigen-specific CD8+ T-cell responses.lld:pubmed
pubmed-article:10203037pubmed:affiliationMolecular Immunology, Institute of Molecular Medicine, Oxford, UK. gogg@worf.molbiol.ox.ac.uklld:pubmed
pubmed-article:10203037pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:10203037pubmed:publicationTypeReviewlld:pubmed
pubmed-article:10203037pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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