| pubmed-article:10195754 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10195754 | lifeskim:mentions | umls-concept:C0001175 | lld:lifeskim |
| pubmed-article:10195754 | lifeskim:mentions | umls-concept:C0042749 | lld:lifeskim |
| pubmed-article:10195754 | lifeskim:mentions | umls-concept:C0032136 | lld:lifeskim |
| pubmed-article:10195754 | lifeskim:mentions | umls-concept:C0024400 | lld:lifeskim |
| pubmed-article:10195754 | lifeskim:mentions | umls-concept:C0012854 | lld:lifeskim |
| pubmed-article:10195754 | lifeskim:mentions | umls-concept:C0042196 | lld:lifeskim |
| pubmed-article:10195754 | lifeskim:mentions | umls-concept:C0679058 | lld:lifeskim |
| pubmed-article:10195754 | lifeskim:mentions | umls-concept:C1547699 | lld:lifeskim |
| pubmed-article:10195754 | lifeskim:mentions | umls-concept:C0442117 | lld:lifeskim |
| pubmed-article:10195754 | lifeskim:mentions | umls-concept:C2700640 | lld:lifeskim |
| pubmed-article:10195754 | lifeskim:mentions | umls-concept:C0449435 | lld:lifeskim |
| pubmed-article:10195754 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:10195754 | pubmed:dateCreated | 1999-7-16 | lld:pubmed |
| pubmed-article:10195754 | pubmed:abstractText | We have succeeded in stably maintaining the entire genome of SIVmac239 as a plasmid clone. Supercoiled proviral plasmid DNA was inoculated intramuscularly into two adult rhesus macaques and into a neonate. All three animals became viremic and seroconverted. Viral kinetics were followed prospectively by quantitative competitive reverse transcriptase polymerase chain reaction (QC-RT-PCR), measurement of proviral DNA load in peripheral blood mononuclear cells (PBMCs) by PCR, and virus isolation by cocultivation. The infant developed high virus loads and succumbed to AIDS and SIV-associated nephropathy at 10 weeks postinoculation. Both adults are still living but have progressed to AIDS; one adult has also developed severe thrombocytopenia. We conclude that infection through intramuscular inoculation of cloned plasmid DNA encoding the entire proviral genome is reproducible and will provide a useful tool for studying viral pathogenesis. | lld:pubmed |
| pubmed-article:10195754 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10195754 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10195754 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10195754 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10195754 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10195754 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10195754 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:10195754 | pubmed:issn | 0889-2229 | lld:pubmed |
| pubmed-article:10195754 | pubmed:author | pubmed-author:LiskaVV | lld:pubmed |
| pubmed-article:10195754 | pubmed:author | pubmed-author:RuprechtR MRM | lld:pubmed |
| pubmed-article:10195754 | pubmed:author | pubmed-author:VlasakJJ | lld:pubmed |
| pubmed-article:10195754 | pubmed:author | pubmed-author:KhimaniA HAH | lld:pubmed |
| pubmed-article:10195754 | pubmed:author | pubmed-author:Hofmann-Lehma... | lld:pubmed |
| pubmed-article:10195754 | pubmed:author | pubmed-author:FinkA NAN | lld:pubmed |
| pubmed-article:10195754 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10195754 | pubmed:day | 20 | lld:pubmed |
| pubmed-article:10195754 | pubmed:volume | 15 | lld:pubmed |
| pubmed-article:10195754 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10195754 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10195754 | pubmed:pagination | 445-50 | lld:pubmed |
| pubmed-article:10195754 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:10195754 | pubmed:meshHeading | pubmed-meshheading:10195754... | lld:pubmed |
| pubmed-article:10195754 | pubmed:meshHeading | pubmed-meshheading:10195754... | lld:pubmed |
| pubmed-article:10195754 | pubmed:meshHeading | pubmed-meshheading:10195754... | lld:pubmed |
| pubmed-article:10195754 | pubmed:meshHeading | pubmed-meshheading:10195754... | lld:pubmed |
| pubmed-article:10195754 | pubmed:meshHeading | pubmed-meshheading:10195754... | lld:pubmed |
| pubmed-article:10195754 | pubmed:meshHeading | pubmed-meshheading:10195754... | lld:pubmed |
| pubmed-article:10195754 | pubmed:meshHeading | pubmed-meshheading:10195754... | lld:pubmed |
| pubmed-article:10195754 | pubmed:meshHeading | pubmed-meshheading:10195754... | lld:pubmed |
| pubmed-article:10195754 | pubmed:meshHeading | pubmed-meshheading:10195754... | lld:pubmed |
| pubmed-article:10195754 | pubmed:meshHeading | pubmed-meshheading:10195754... | lld:pubmed |
| pubmed-article:10195754 | pubmed:meshHeading | pubmed-meshheading:10195754... | lld:pubmed |
| pubmed-article:10195754 | pubmed:meshHeading | pubmed-meshheading:10195754... | lld:pubmed |
| pubmed-article:10195754 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10195754 | pubmed:articleTitle | Viremia and AIDS in rhesus macaques after intramuscular inoculation of plasmid DNA encoding full-length SIVmac239. | lld:pubmed |
| pubmed-article:10195754 | pubmed:affiliation | Laboratory of Viral Pathogenesis, Dana-Farber Cancer Institute, Boston, Massachusetts 02115, USA. | lld:pubmed |
| pubmed-article:10195754 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10195754 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:10195754 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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