Linked Life Data

1009576

Source:http://linkedlifedata.com/resource/pubmed/id/1009576

Statements in which the resource exists.
SubjectPredicateObjectContext
pubmed-article:1009576rdf:typepubmed:Citationlld:pubmed
pubmed-article:1009576lifeskim:mentionsumls-concept:C0012939lld:lifeskim
pubmed-article:1009576lifeskim:mentionsumls-concept:C0598312lld:lifeskim
pubmed-article:1009576lifeskim:mentionsumls-concept:C0162326lld:lifeskim
pubmed-article:1009576lifeskim:mentionsumls-concept:C0884118lld:lifeskim
pubmed-article:1009576lifeskim:mentionsumls-concept:C1880022lld:lifeskim
pubmed-article:1009576pubmed:issue4 Pt 1lld:pubmed
pubmed-article:1009576pubmed:dateCreated1977-3-15lld:pubmed
pubmed-article:1009576pubmed:abstractTextReplication intermediates of adenovirus DNA apparently contain extensive stretches of single-stranded DNA. Such single-stranded viral DNA sequences homologous to different regions of the viral genome present in adenovirus-infected cells during viral DNA replication have therefore been characterized by hybridization to the separated strands of restriction endonuclease fragments of 32P-labeled adenovirus types 2 and 5 DNA. Saturation hybridization experiments with infected cell DNA extracted at late times suggest that all regions of the adenovirus genome are represented in the single-stranded fraction, but at unequal frequencies. This nonuniform representation has been characterized in more detail with self-annealed, total cell DNA extracted 18 hr after adenovirus type 2 infection: the concentration of single-stranded sequences homologous to different regions of the viral genome was determined by comparing the rates of hybridization of 32P-labeled, single-stranded DNA probes with such self-annealed 18 hr DNA to the rates of hybridization of the same probes with equal concentrations of their complements. This approach allows the concentration of single-stranded viral DNA sequences in excess of their complements to be determined. Such sequences can be represented by two concentration gradients across the viral genome: those homologous to the r strand increase in concentration from 27.8-40.9 units toward the right end, whereas sequences homologous to the 1 strand increase from an area 27.8-40.9 units toward the left end. The time course of synthesis of single-stranded viral DNA sequences relative to accumulation of total viral DNA during the productive cycle and their behavior following a shift of H5ts125-infected cells in which viral DNA replication has begun from a permissive to a nonpermissive temperature support the contention that these sequences are indeed generated as adenovirus DNA is replicated. These results are therefore discussed in terms of current models of adenovirus DNA replication.lld:pubmed
pubmed-article:1009576pubmed:languageenglld:pubmed
pubmed-article:1009576pubmed:journalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:1009576pubmed:citationSubsetIMlld:pubmed
pubmed-article:1009576pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:1009576pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:1009576pubmed:statusMEDLINElld:pubmed
pubmed-article:1009576pubmed:monthDeclld:pubmed
pubmed-article:1009576pubmed:issn0092-8674lld:pubmed
pubmed-article:1009576pubmed:authorpubmed-author:SharpP APAlld:pubmed
pubmed-article:1009576pubmed:authorpubmed-author:BergenS SSSlld:pubmed
pubmed-article:1009576pubmed:authorpubmed-author:FlintS JSJlld:pubmed
pubmed-article:1009576pubmed:issnTypePrintlld:pubmed
pubmed-article:1009576pubmed:volume9lld:pubmed
pubmed-article:1009576pubmed:ownerNLMlld:pubmed
pubmed-article:1009576pubmed:authorsCompleteYlld:pubmed
pubmed-article:1009576pubmed:pagination559-71lld:pubmed
pubmed-article:1009576pubmed:dateRevised2006-11-15lld:pubmed
pubmed-article:1009576pubmed:meshHeadingpubmed-meshheading:1009576-...lld:pubmed
pubmed-article:1009576pubmed:meshHeadingpubmed-meshheading:1009576-...lld:pubmed
pubmed-article:1009576pubmed:meshHeadingpubmed-meshheading:1009576-...lld:pubmed
pubmed-article:1009576pubmed:meshHeadingpubmed-meshheading:1009576-...lld:pubmed
pubmed-article:1009576pubmed:meshHeadingpubmed-meshheading:1009576-...lld:pubmed
pubmed-article:1009576pubmed:meshHeadingpubmed-meshheading:1009576-...lld:pubmed
pubmed-article:1009576pubmed:meshHeadingpubmed-meshheading:1009576-...lld:pubmed
pubmed-article:1009576pubmed:meshHeadingpubmed-meshheading:1009576-...lld:pubmed
pubmed-article:1009576pubmed:year1976lld:pubmed
pubmed-article:1009576pubmed:articleTitleCharacterization of single-stranded viral DNA sequences present during replication of adenovirus types 2 and 5.lld:pubmed
pubmed-article:1009576pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:1009576pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:1009576lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:1009576lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:1009576lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:1009576lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:1009576lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:1009576lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:1009576lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:1009576lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:1009576lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:1009576lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:1009576lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:1009576lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:1009576lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:1009576lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:1009576lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:1009576lld:pubmed