| pubmed-article:10092775 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10092775 | lifeskim:mentions | umls-concept:C0682523 | lld:lifeskim |
| pubmed-article:10092775 | lifeskim:mentions | umls-concept:C0026336 | lld:lifeskim |
| pubmed-article:10092775 | lifeskim:mentions | umls-concept:C0870134 | lld:lifeskim |
| pubmed-article:10092775 | lifeskim:mentions | umls-concept:C0007589 | lld:lifeskim |
| pubmed-article:10092775 | lifeskim:mentions | umls-concept:C0011306 | lld:lifeskim |
| pubmed-article:10092775 | lifeskim:mentions | umls-concept:C1332710 | lld:lifeskim |
| pubmed-article:10092775 | lifeskim:mentions | umls-concept:C2755994 | lld:lifeskim |
| pubmed-article:10092775 | lifeskim:mentions | umls-concept:C0332120 | lld:lifeskim |
| pubmed-article:10092775 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:10092775 | pubmed:dateCreated | 1999-4-13 | lld:pubmed |
| pubmed-article:10092775 | pubmed:abstractText | Intracellular signals that mediate differentiation of pluripotent hemopoietic progenitors to dendritic cells (DC) are largely undefined. We have previously shown that protein kinase C (PKC) activation (with phorbol ester (PMA) alone) specifically induces differentiation of primary human CD34+ hemopoietic progenitor cells (HPC) to mature DC. We now find that cytokine-driven (granulocyte-macrophage CSF and TNF-alpha) CD34+ HPC-->DC differentiation is preferentially blocked by inhibitors of PKC activation. To further identify intracellular signals and downstream events important in CD34+ HPC-->DC differentiation we have characterized a human leukemic cell line model of this process. The CD34+ myelomonocytic cell line KG1 differentiates into dendritic-like cells in response to granulocyte-macrophage CSF plus TNF-alpha, or PMA (with or without the calcium ionophore ionomycin, or TNF-alpha), with different stimuli mediating different aspects of the process. Phenotypic DC characteristics of KG1 dendritic-like cells include morphology (loosely adherent cells with long neurite processes), MHC I+/MHC IIbright/CD83+/CD86+/CD14- surface Ag expression, and RelB and DC-CK1 gene expression. Functional DC characteristics include fluid phase macromolecule uptake (FITC-dextran) and activation of resting T cells. Comparison of KG1 to the PMA-unresponsive subline KG1a reveals differences in expression of TNF receptors 1 and 2; PKC isoforms alpha, beta I, beta II, and mu; and RelB, suggesting that these components/pathways are important for DC differentiation. Together, these findings demonstrate that cytokine or phorbol ester stimulation of KG1 is a model of human CD34+ HPC to DC differentiation and suggest that specific intracellular signaling pathways mediate specific events in DC lineage commitment. | lld:pubmed |
| pubmed-article:10092775 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10092775 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:10092775 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:10092775 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:10092775 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10092775 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:10092775 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10092775 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:10092775 | pubmed:issn | 0022-1767 | lld:pubmed |
| pubmed-article:10092775 | pubmed:author | pubmed-author:WilliamsA JAJ | lld:pubmed |
| pubmed-article:10092775 | pubmed:author | pubmed-author:LECHWW | lld:pubmed |
| pubmed-article:10092775 | pubmed:author | pubmed-author:WellsM RMR | lld:pubmed |
| pubmed-article:10092775 | pubmed:author | pubmed-author:GUYL PLP | lld:pubmed |
| pubmed-article:10092775 | pubmed:author | pubmed-author:SiebenlistUU | lld:pubmed |
| pubmed-article:10092775 | pubmed:author | pubmed-author:KaushalSS | lld:pubmed |
| pubmed-article:10092775 | pubmed:author | pubmed-author:HarlanD MDM | lld:pubmed |
| pubmed-article:10092775 | pubmed:author | pubmed-author:CarlsonL MLM | lld:pubmed |
| pubmed-article:10092775 | pubmed:author | pubmed-author:BlairP JPJ | lld:pubmed |
| pubmed-article:10092775 | pubmed:author | pubmed-author:FranzosoGG | lld:pubmed |
| pubmed-article:10092775 | pubmed:author | pubmed-author:GrimesJ LJL | lld:pubmed |
| pubmed-article:10092775 | pubmed:author | pubmed-author:MurilloMM | lld:pubmed |
| pubmed-article:10092775 | pubmed:author | pubmed-author:ChuteJ PJP | lld:pubmed |
| pubmed-article:10092775 | pubmed:author | pubmed-author:SmootD SDS | lld:pubmed |
| pubmed-article:10092775 | pubmed:author | pubmed-author:St LouisD CDC | lld:pubmed |
| pubmed-article:10092775 | pubmed:author | pubmed-author:WoodcockJ BJB | lld:pubmed |
| pubmed-article:10092775 | pubmed:author | pubmed-author:FransozoGG | lld:pubmed |
| pubmed-article:10092775 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10092775 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:10092775 | pubmed:volume | 162 | lld:pubmed |
| pubmed-article:10092775 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10092775 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10092775 | pubmed:pagination | 3237-48 | lld:pubmed |
| pubmed-article:10092775 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
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| pubmed-article:10092775 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10092775 | pubmed:articleTitle | Evidence for distinct intracellular signaling pathways in CD34+ progenitor to dendritic cell differentiation from a human cell line model. | lld:pubmed |
| pubmed-article:10092775 | pubmed:affiliation | Immune Cell Biology Program, Naval Medical Research Institute, Bethesda, MD 20889, USA. | lld:pubmed |
| pubmed-article:10092775 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10092775 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
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