| pubmed-article:10092170 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10092170 | lifeskim:mentions | umls-concept:C0330189 | lld:lifeskim |
| pubmed-article:10092170 | lifeskim:mentions | umls-concept:C0949850 | lld:lifeskim |
| pubmed-article:10092170 | lifeskim:mentions | umls-concept:C0996599 | lld:lifeskim |
| pubmed-article:10092170 | lifeskim:mentions | umls-concept:C1510411 | lld:lifeskim |
| pubmed-article:10092170 | lifeskim:mentions | umls-concept:C0028423 | lld:lifeskim |
| pubmed-article:10092170 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:10092170 | pubmed:dateCreated | 1999-4-13 | lld:pubmed |
| pubmed-article:10092170 | pubmed:abstractText | Agrobacterium-mediated gene transfer is the method of choice for many plant biotechnology laboratories; however, large-scale use of this organism in conifer transformation has been limited by difficult propagation of explant material, selection efficiencies and low transformation frequency. We have analyzed co-cultivation conditions and different disarmed strains of Agrobacterium to improve transformation. Additional copies of virulence genes were added to three common disarmed strains. These extra virulence genes included either a constitutively active virG or extra copies of virG and virB, both from pTiBo542. In experiments with Norway spruce, we increased transformation efficiencies 1000-fold from initial experiments where little or no transient expression was detected. Over 100 transformed lines expressing the marker gene beta-glucuronidase (GUS) were generated from rapidly dividing embryogenic suspension-cultured cells co-cultivated with Agrobacterium. GUS activity was used to monitor transient expression and to further test lines selected on kanamycin-containing medium. In loblolly pine, transient expression increased 10-fold utilizing modified Agrobacterium strains. Agrobacterium-mediated gene transfer is a useful technique for large-scale generation of transgenic Norway spruce and may prove useful for other conifer species. | lld:pubmed |
| pubmed-article:10092170 | pubmed:keyword | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10092170 | pubmed:keyword | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10092170 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10092170 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10092170 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10092170 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10092170 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10092170 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10092170 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10092170 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10092170 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:10092170 | pubmed:issn | 0167-4412 | lld:pubmed |
| pubmed-article:10092170 | pubmed:author | pubmed-author:QuinnMM | lld:pubmed |
| pubmed-article:10092170 | pubmed:author | pubmed-author:SederoffRR | lld:pubmed |
| pubmed-article:10092170 | pubmed:author | pubmed-author:WenckA RAR | lld:pubmed |
| pubmed-article:10092170 | pubmed:author | pubmed-author:WhettenR WRW | lld:pubmed |
| pubmed-article:10092170 | pubmed:author | pubmed-author:PullmanGG | lld:pubmed |
| pubmed-article:10092170 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10092170 | pubmed:volume | 39 | lld:pubmed |
| pubmed-article:10092170 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10092170 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10092170 | pubmed:pagination | 407-16 | lld:pubmed |
| pubmed-article:10092170 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
| pubmed-article:10092170 | pubmed:meshHeading | pubmed-meshheading:10092170... | lld:pubmed |
| pubmed-article:10092170 | pubmed:meshHeading | pubmed-meshheading:10092170... | lld:pubmed |
| pubmed-article:10092170 | pubmed:meshHeading | pubmed-meshheading:10092170... | lld:pubmed |
| pubmed-article:10092170 | pubmed:meshHeading | pubmed-meshheading:10092170... | lld:pubmed |
| pubmed-article:10092170 | pubmed:meshHeading | pubmed-meshheading:10092170... | lld:pubmed |
| pubmed-article:10092170 | pubmed:meshHeading | pubmed-meshheading:10092170... | lld:pubmed |
| pubmed-article:10092170 | pubmed:meshHeading | pubmed-meshheading:10092170... | lld:pubmed |
| pubmed-article:10092170 | pubmed:meshHeading | pubmed-meshheading:10092170... | lld:pubmed |
| pubmed-article:10092170 | pubmed:meshHeading | pubmed-meshheading:10092170... | lld:pubmed |
| pubmed-article:10092170 | pubmed:meshHeading | pubmed-meshheading:10092170... | lld:pubmed |
| pubmed-article:10092170 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10092170 | pubmed:articleTitle | High-efficiency Agrobacterium-mediated transformation of Norway spruce (Picea abies) and loblolly pine (Pinus taeda). | lld:pubmed |
| pubmed-article:10092170 | pubmed:affiliation | Forest Biotechnology Group, North Carolina State University, Raleigh 27695, USA. | lld:pubmed |
| pubmed-article:10092170 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10092170 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:10092170 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10092170 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10092170 | lld:pubmed |
