| pubmed-article:10092103 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10092103 | lifeskim:mentions | umls-concept:C0020971 | lld:lifeskim |
| pubmed-article:10092103 | lifeskim:mentions | umls-concept:C0004611 | lld:lifeskim |
| pubmed-article:10092103 | lifeskim:mentions | umls-concept:C0026724 | lld:lifeskim |
| pubmed-article:10092103 | lifeskim:mentions | umls-concept:C0012854 | lld:lifeskim |
| pubmed-article:10092103 | lifeskim:mentions | umls-concept:C1545588 | lld:lifeskim |
| pubmed-article:10092103 | lifeskim:mentions | umls-concept:C0205373 | lld:lifeskim |
| pubmed-article:10092103 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:10092103 | pubmed:dateCreated | 1999-4-21 | lld:pubmed |
| pubmed-article:10092103 | pubmed:abstractText | Naked plasmid DNA (pRc/Y-hsp60) with a cytomegalovirus promoter and a sequence encoding Yersinia enterocolitica 60-kDa heat shock protein (Y-HSP60) was used for vaccination. After intramuscular injection of pRc/Y-hsp60, Y-hsp60 mRNA could be detected by reverse transcription-PCR in muscle, liver and spleen. A single immunization with pRc/Y-hsp60 induced significant Y-HSP60-specific T cell responses after 1 week. IFN-gamma production by spleen cells upon stimulation with Y-HSP60 was strictly dependent on the presence of CD4+ T cells, indicating the generation of a Th1 response upon DNA immunization. DNA immunization in addition induced strong Y-HSP60-specific IgG2a, weak IgG1, but not IgA antibodies. Immunization of BALB/c and C57BL/6 mice with pRc/Y-hsp60 conferred protection against disseminated Y. enterocolitica infection in spleen, but not at the site of mucosal entry, the Peyer's patches. Furthermore, pRc/Y-hsp60 vaccination did not induce cross-protection against related pathogens. Vaccination of beta2-microglobulin- and H2-I-Abeta-deficient mice was not protective, suggesting that both CD4+ and CD8+ T cells are required for protective immunity induced by DNA vaccination. | lld:pubmed |
| pubmed-article:10092103 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10092103 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10092103 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10092103 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10092103 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10092103 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10092103 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10092103 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10092103 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10092103 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10092103 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10092103 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:10092103 | pubmed:issn | 0014-2980 | lld:pubmed |
| pubmed-article:10092103 | pubmed:author | pubmed-author:BohnEE | lld:pubmed |
| pubmed-article:10092103 | pubmed:author | pubmed-author:ReimannJJ | lld:pubmed |
| pubmed-article:10092103 | pubmed:author | pubmed-author:NollAA | lld:pubmed |
| pubmed-article:10092103 | pubmed:author | pubmed-author:AutenriethI... | lld:pubmed |
| pubmed-article:10092103 | pubmed:author | pubmed-author:SchirmbeckRR | lld:pubmed |
| pubmed-article:10092103 | pubmed:author | pubmed-author:BüchelerNN | lld:pubmed |
| pubmed-article:10092103 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10092103 | pubmed:volume | 29 | lld:pubmed |
| pubmed-article:10092103 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10092103 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10092103 | pubmed:pagination | 986-96 | lld:pubmed |
| pubmed-article:10092103 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
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| pubmed-article:10092103 | pubmed:meshHeading | pubmed-meshheading:10092103... | lld:pubmed |
| pubmed-article:10092103 | pubmed:meshHeading | pubmed-meshheading:10092103... | lld:pubmed |
| pubmed-article:10092103 | pubmed:meshHeading | pubmed-meshheading:10092103... | lld:pubmed |
| pubmed-article:10092103 | pubmed:meshHeading | pubmed-meshheading:10092103... | lld:pubmed |
| pubmed-article:10092103 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10092103 | pubmed:articleTitle | DNA immunization confers systemic, but not mucosal, protection against enteroinvasive bacteria. | lld:pubmed |
| pubmed-article:10092103 | pubmed:affiliation | Max von Pettenkofer-Institut für Hygiene und Medizinische Mikrobiologie, LMU München, Germany. | lld:pubmed |
| pubmed-article:10092103 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10092103 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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