pubmed-article:1009107 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:1009107 | lifeskim:mentions | umls-concept:C0018557 | lld:lifeskim |
pubmed-article:1009107 | lifeskim:mentions | umls-concept:C0040578 | lld:lifeskim |
pubmed-article:1009107 | lifeskim:mentions | umls-concept:C0029205 | lld:lifeskim |
pubmed-article:1009107 | lifeskim:mentions | umls-concept:C0596625 | lld:lifeskim |
pubmed-article:1009107 | pubmed:issue | 1 | lld:pubmed |
pubmed-article:1009107 | pubmed:dateCreated | 1977-3-31 | lld:pubmed |
pubmed-article:1009107 | pubmed:abstractText | Conditions have been developed for maintaining hamster tracheas in organ culture for at least 10 days. Secreted glycoproteins labelled with [14C]glucosamine and [3H]fucose were isolated from the spent medium and digested with papain, and the digest was fractionated on DEAE-Sephadex by stepwise elution with NaCl. The fractions eluted by 0.1 and 0.2 M NaCl and some of the products eluted with 0.4 M NaCl were shown to be derived from epithelial glycoproteins. Glycosaminoglycans were eluted by 0.4 M and by 1.25 M NaCl. Glycopeptides isolated from the epithelium by homogenization, ethanol precipitation and papain digestion, and defined as "intracellular", gave a very similar profile on DEAE-Sephadex. The 0.1 M glycopeptide peak was the major fraction of epithelial origin from both secreted and "intracellular" material; it labelled extensively with both glucosamine and fucose and had a molecular fraction was purified further by chromatography on Sephadex G-75 and DEAE-Sephadex; its amino acid and carbohydrate compositions were determined. | lld:pubmed |
pubmed-article:1009107 | pubmed:language | eng | lld:pubmed |
pubmed-article:1009107 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:1009107 | pubmed:citationSubset | IM | lld:pubmed |
pubmed-article:1009107 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:1009107 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:1009107 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:1009107 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:1009107 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:1009107 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:1009107 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:1009107 | pubmed:month | Nov | lld:pubmed |
pubmed-article:1009107 | pubmed:issn | 0006-3002 | lld:pubmed |
pubmed-article:1009107 | pubmed:author | pubmed-author:WolfGG | lld:pubmed |
pubmed-article:1009107 | pubmed:author | pubmed-author:DanielP FPF | lld:pubmed |
pubmed-article:1009107 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:1009107 | pubmed:day | 18 | lld:pubmed |
pubmed-article:1009107 | pubmed:volume | 451 | lld:pubmed |
pubmed-article:1009107 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:1009107 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:1009107 | pubmed:pagination | 184-200 | lld:pubmed |
pubmed-article:1009107 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
pubmed-article:1009107 | pubmed:meshHeading | pubmed-meshheading:1009107-... | lld:pubmed |
pubmed-article:1009107 | pubmed:meshHeading | pubmed-meshheading:1009107-... | lld:pubmed |
pubmed-article:1009107 | pubmed:meshHeading | pubmed-meshheading:1009107-... | lld:pubmed |
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pubmed-article:1009107 | pubmed:meshHeading | pubmed-meshheading:1009107-... | lld:pubmed |
pubmed-article:1009107 | pubmed:meshHeading | pubmed-meshheading:1009107-... | lld:pubmed |
pubmed-article:1009107 | pubmed:meshHeading | pubmed-meshheading:1009107-... | lld:pubmed |
pubmed-article:1009107 | pubmed:year | 1976 | lld:pubmed |
pubmed-article:1009107 | pubmed:articleTitle | Glycoprotein biosynthesis by organ cultures of hamster trachea. | lld:pubmed |
pubmed-article:1009107 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:1009107 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1009107 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1009107 | lld:pubmed |