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pubmed-article:10089179pubmed:abstractTextShort transverse relaxation times of Calpha and Cbeta single-quantum coherences reduce the sensitivity of triple-resonance experiments involving transfers of Calpha/Cbeta or Halpha/Hbeta coherences. Multiple-quantum line-narrowing techniques improve the relaxation properties of 13C coherences, thereby increasing the sensitivity of the experiment. In the present work, we describe PFG-CBCA(CO)NH and PFG-HBHA(CO)NH experiments that utilize heteronuclear multiple-quantum coherences in a simultaneous constant-time period to obtain completely decoupled spectra with improved sensitivity. Results indicate that approximately 30% of cross peaks show an average enhancement of approximately 15% in the CBCA(CO)NH experiment. In the related HBHA(CO)NH experiment, approximately 97% of the cross peaks show an average enhancement of approximately 40%.lld:pubmed
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pubmed-article:10089179pubmed:authorpubmed-author:MontelioneG...lld:pubmed
pubmed-article:10089179pubmed:authorpubmed-author:SwapnaG VGVlld:pubmed
pubmed-article:10089179pubmed:copyrightInfoCopyright 1999 Academic Press.lld:pubmed
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pubmed-article:10089179pubmed:volume137lld:pubmed
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pubmed-article:10089179pubmed:pagination437-42lld:pubmed
pubmed-article:10089179pubmed:dateRevised2007-11-14lld:pubmed
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pubmed-article:10089179pubmed:year1999lld:pubmed
pubmed-article:10089179pubmed:articleTitleSensitivity-enhanced sim-CT HMQC PFG-HBHA(CO)NH and PFG-CBCA(CO)NH triple-resonance experiments.lld:pubmed
pubmed-article:10089179pubmed:affiliationDepartment of Molecular Biology and Biochemistry, Rutgers University, Piscataway, New Jersey, 08854-5638, USA. guy@nmrlab.cabm.rutgers.edulld:pubmed
pubmed-article:10089179pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:10089179pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
pubmed-article:10089179pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed