| pubmed-article:10088944 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10088944 | lifeskim:mentions | umls-concept:C0019740 | lld:lifeskim |
| pubmed-article:10088944 | lifeskim:mentions | umls-concept:C0079247 | lld:lifeskim |
| pubmed-article:10088944 | lifeskim:mentions | umls-concept:C0039593 | lld:lifeskim |
| pubmed-article:10088944 | lifeskim:mentions | umls-concept:C0004083 | lld:lifeskim |
| pubmed-article:10088944 | lifeskim:mentions | umls-concept:C0016263 | lld:lifeskim |
| pubmed-article:10088944 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:10088944 | pubmed:dateCreated | 1999-5-4 | lld:pubmed |
| pubmed-article:10088944 | pubmed:abstractText | HLA-B27 typing contributes to the diagnosis of ankylosing spondylitis. The classical technique of microlymphocytotoxicity is costly and can give false-negative results. We have compared 304 samples using two relatively new methods - flow cytometry and PCR-SSP - and evaluated their respective uses in routine analysis. Flow cytometric HLA-B27 testing was performed using three monoclonal anti-B27 antibodies (HLA-ABC-m3, GS145.2 and FD705 clones). Cut-off values were established to differentiate HLA-B27-positive from HLA-B27-negative samples with ROC curves. Although flow cytometric analysis with a reliable monoclonal antibody (mAb) is valuable for HLA screening, none of the HLA-B27 flow cytometry protocols was sufficient on its own to ascertain the HLA phenotype in 100% of samples. Two false negatives were observed with the FD705 mAb and the use of two different monoclonal antibodies did not increase the accuracy of HLA-B27 typing. HLA-B27 typing using molecular biology is a reliable but costly technique. Therefore we suggest that DNA typing could be used as a complementary technique and applied to samples whose HLA-B27 phenotype cannot be determined by flow cytometry. The association of flow cytometry and DNA typing is, in our experience, an economical and reliable approach. | lld:pubmed |
| pubmed-article:10088944 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10088944 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10088944 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10088944 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10088944 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10088944 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10088944 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10088944 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10088944 | pubmed:issn | 0770-3198 | lld:pubmed |
| pubmed-article:10088944 | pubmed:author | pubmed-author:BonnaudGG | lld:pubmed |
| pubmed-article:10088944 | pubmed:author | pubmed-author:DrouetMM | lld:pubmed |
| pubmed-article:10088944 | pubmed:author | pubmed-author:CognéMM | lld:pubmed |
| pubmed-article:10088944 | pubmed:author | pubmed-author:PreuxP MPM | lld:pubmed |
| pubmed-article:10088944 | pubmed:author | pubmed-author:AupetitCC | lld:pubmed |
| pubmed-article:10088944 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10088944 | pubmed:volume | 18 | lld:pubmed |
| pubmed-article:10088944 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10088944 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10088944 | pubmed:pagination | 23-7 | lld:pubmed |
| pubmed-article:10088944 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:10088944 | pubmed:meshHeading | pubmed-meshheading:10088944... | lld:pubmed |
| pubmed-article:10088944 | pubmed:meshHeading | pubmed-meshheading:10088944... | lld:pubmed |
| pubmed-article:10088944 | pubmed:meshHeading | pubmed-meshheading:10088944... | lld:pubmed |
| pubmed-article:10088944 | pubmed:meshHeading | pubmed-meshheading:10088944... | lld:pubmed |
| pubmed-article:10088944 | pubmed:meshHeading | pubmed-meshheading:10088944... | lld:pubmed |
| pubmed-article:10088944 | pubmed:meshHeading | pubmed-meshheading:10088944... | lld:pubmed |
| pubmed-article:10088944 | pubmed:meshHeading | pubmed-meshheading:10088944... | lld:pubmed |
| pubmed-article:10088944 | pubmed:meshHeading | pubmed-meshheading:10088944... | lld:pubmed |
| pubmed-article:10088944 | pubmed:meshHeading | pubmed-meshheading:10088944... | lld:pubmed |
| pubmed-article:10088944 | pubmed:meshHeading | pubmed-meshheading:10088944... | lld:pubmed |
| pubmed-article:10088944 | pubmed:meshHeading | pubmed-meshheading:10088944... | lld:pubmed |
| pubmed-article:10088944 | pubmed:meshHeading | pubmed-meshheading:10088944... | lld:pubmed |
| pubmed-article:10088944 | pubmed:meshHeading | pubmed-meshheading:10088944... | lld:pubmed |
| pubmed-article:10088944 | pubmed:meshHeading | pubmed-meshheading:10088944... | lld:pubmed |
| pubmed-article:10088944 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10088944 | pubmed:articleTitle | Optimisation of HLA-B27 testing by association of flow cytometry and DNA typing. | lld:pubmed |
| pubmed-article:10088944 | pubmed:affiliation | Laboratoire d'Immunologie et Immunogénétique, CHRU Dupuytren, Limoges, France. | lld:pubmed |
| pubmed-article:10088944 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10088944 | pubmed:publicationType | Clinical Trial | lld:pubmed |
| pubmed-article:10088944 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:10088944 | pubmed:publicationType | Randomized Controlled Trial | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10088944 | lld:pubmed |
