| pubmed-article:10087149 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10087149 | lifeskim:mentions | umls-concept:C0034493 | lld:lifeskim |
| pubmed-article:10087149 | lifeskim:mentions | umls-concept:C0007776 | lld:lifeskim |
| pubmed-article:10087149 | lifeskim:mentions | umls-concept:C0022655 | lld:lifeskim |
| pubmed-article:10087149 | lifeskim:mentions | umls-concept:C0001613 | lld:lifeskim |
| pubmed-article:10087149 | lifeskim:mentions | umls-concept:C0015385 | lld:lifeskim |
| pubmed-article:10087149 | lifeskim:mentions | umls-concept:C0249742 | lld:lifeskim |
| pubmed-article:10087149 | lifeskim:mentions | umls-concept:C0031669 | lld:lifeskim |
| pubmed-article:10087149 | lifeskim:mentions | umls-concept:C1948027 | lld:lifeskim |
| pubmed-article:10087149 | lifeskim:mentions | umls-concept:C0205385 | lld:lifeskim |
| pubmed-article:10087149 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:10087149 | pubmed:dateCreated | 1999-6-4 | lld:pubmed |
| pubmed-article:10087149 | pubmed:abstractText | The recently cloned rabbit kidney Ca2+-sensing receptor (RabCaR) was functionally characterized in microperfused rabbit cortical thick ascending limb (CTAL) segments. Reverse transcriptase polymerase chain reaction (RT-PCR) confirmed that this nephron segment contains mRNAs coding for the RabCaR. Elevation of the extracellular Ca2+ concentration ([Ca2+]e) from 1 to 5 mmol l-1 induced an increase in the fluorescence emission ratio (R), thus reflecting an increase in intracellular Ca2+ activity ([Ca2+]i). This increase was inhibited by verapamil, nifedipine and SKF 96365, and potentiated by a previous application of Bay K 8644. Neither verapamil nor Bay K 8644 modified the resting [Ca2+]i. This suggests that the basolateral Ca2+ influx induced by a high [Ca2+]e occurs via verapamil- and dihydropyridine-sensitive Ca2+ channels, which are not open under resting conditions. In contrast to that evoked by antidiuretic hormone (ADH), the [Ca2+]i increase induced by a high [Ca2+]e did not result from an accumulation of inositol phosphates. Neomycin, Gd3+, Mg2+, commonly used agonists of the Ca2+-sensing receptor, did not increase the [Ca2+]i. In the presence of verapamil, ADH still produced a transient [Ca2+]i increase that was not observed in the presence of an increased [Ca2+]e. These results suggest that the RabCaR in rabbit CTAL cells is not functionally coupled to phospholipase C. In conclusion, the high [Ca2+]e-induced [Ca2+]i increase involves verapamil- and dihydropyridine-sensitive Ca2+ channels and is independent of phosphoinositide metabolism. Whether these channels are activated by the RabCaR remains to be elucidated. | lld:pubmed |
| pubmed-article:10087149 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10087149 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10087149 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10087149 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10087149 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10087149 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10087149 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10087149 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10087149 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10087149 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10087149 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10087149 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10087149 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10087149 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10087149 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10087149 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:10087149 | pubmed:issn | 0031-6768 | lld:pubmed |
| pubmed-article:10087149 | pubmed:author | pubmed-author:NitschkeRR | lld:pubmed |
| pubmed-article:10087149 | pubmed:author | pubmed-author:Di StefanoAA | lld:pubmed |
| pubmed-article:10087149 | pubmed:author | pubmed-author:WittnerMM | lld:pubmed |
| pubmed-article:10087149 | pubmed:author | pubmed-author:RajerisonR... | lld:pubmed |
| pubmed-article:10087149 | pubmed:author | pubmed-author:PallasJ EJEJr | lld:pubmed |
| pubmed-article:10087149 | pubmed:author | pubmed-author:DesfleursEE | lld:pubmed |
| pubmed-article:10087149 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10087149 | pubmed:volume | 437 | lld:pubmed |
| pubmed-article:10087149 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10087149 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10087149 | pubmed:pagination | 716-23 | lld:pubmed |
| pubmed-article:10087149 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
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| pubmed-article:10087149 | pubmed:meshHeading | pubmed-meshheading:10087149... | lld:pubmed |
| pubmed-article:10087149 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10087149 | pubmed:articleTitle | The Ca2+-sensing receptor in the rabbit cortical thick ascending limb (CTAL) is functionally not coupled to phospholipase C. | lld:pubmed |
| pubmed-article:10087149 | pubmed:affiliation | Département de Biologie Cellulaire et Moléculaire, CEA Saclay, URA CNRS 1859, F-91191 Gif-sur-Yvette, France. | lld:pubmed |
| pubmed-article:10087149 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10087149 | pubmed:publicationType | In Vitro | lld:pubmed |
| pubmed-article:10087149 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10087149 | lld:pubmed |
