| pubmed-article:10082387 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10082387 | lifeskim:mentions | umls-concept:C0011139 | lld:lifeskim |
| pubmed-article:10082387 | lifeskim:mentions | umls-concept:C0014665 | lld:lifeskim |
| pubmed-article:10082387 | lifeskim:mentions | umls-concept:C0017263 | lld:lifeskim |
| pubmed-article:10082387 | lifeskim:mentions | umls-concept:C0017376 | lld:lifeskim |
| pubmed-article:10082387 | lifeskim:mentions | umls-concept:C2700640 | lld:lifeskim |
| pubmed-article:10082387 | lifeskim:mentions | umls-concept:C0598148 | lld:lifeskim |
| pubmed-article:10082387 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:10082387 | pubmed:dateCreated | 1999-4-23 | lld:pubmed |
| pubmed-article:10082387 | pubmed:abstractText | The genomes of equine herpesvirus 1 (EHV-1) defective interfering (DI) particles that mediate persistent infection were shown to encode a unique hybrid open reading frame composed of sequences that encode the 196 N-terminal amino acids of ICP22 linked in-frame to the C-terminal 68 amino acids of ICP27. Previous studies demonstrated that this hybrid gene, designated as ICP22/ICP27. was expressed abundantly at both the mRNA and the protein levels in DI particle-enriched infections, but not in standard EHV-1 infection (Chen et al., 1996 J. Virol. 70, 313-320). Since the ICP22/ICP27 hybrid protein contains portions of two EHV-1 early regulatory proteins, its effect on EHV-1 gene regulation was investigated. In EHV-1-infected cells, the ICP22/ICP27 hybrid protein expressed from plasmid vectors significantly reduced expression of a reporter gene under the control of the EHV-1 immediate-early (IE) gene promoter and early gene promoter, such as the viral ICP27 gene. In uninfected cells, the ICP22/ICP27 hybrid protein moderately down-regulated the IE and ICP22 promoters, up-regulated late gene promoters such as IR5, and altered the regulatory function of the IE and 1CP22 proteins in co-transfected cells. These results demonstrated that DI particles might alter viral gene regulation by expression of a unique hybrid gene encoded on the DI particle genome. | lld:pubmed |
| pubmed-article:10082387 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10082387 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10082387 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10082387 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10082387 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10082387 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10082387 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10082387 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10082387 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10082387 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10082387 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10082387 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10082387 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10082387 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:10082387 | pubmed:issn | 0168-1702 | lld:pubmed |
| pubmed-article:10082387 | pubmed:author | pubmed-author:O'CallaghanD... | lld:pubmed |
| pubmed-article:10082387 | pubmed:author | pubmed-author:ChenMM | lld:pubmed |
| pubmed-article:10082387 | pubmed:author | pubmed-author:ZhangYY | lld:pubmed |
| pubmed-article:10082387 | pubmed:author | pubmed-author:Garko-Buczyns... | lld:pubmed |
| pubmed-article:10082387 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10082387 | pubmed:volume | 59 | lld:pubmed |
| pubmed-article:10082387 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10082387 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10082387 | pubmed:pagination | 149-64 | lld:pubmed |
| pubmed-article:10082387 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
| pubmed-article:10082387 | pubmed:meshHeading | pubmed-meshheading:10082387... | lld:pubmed |
| pubmed-article:10082387 | pubmed:meshHeading | pubmed-meshheading:10082387... | lld:pubmed |
| pubmed-article:10082387 | pubmed:meshHeading | pubmed-meshheading:10082387... | lld:pubmed |
| pubmed-article:10082387 | pubmed:meshHeading | pubmed-meshheading:10082387... | lld:pubmed |
| pubmed-article:10082387 | pubmed:meshHeading | pubmed-meshheading:10082387... | lld:pubmed |
| pubmed-article:10082387 | pubmed:meshHeading | pubmed-meshheading:10082387... | lld:pubmed |
| pubmed-article:10082387 | pubmed:meshHeading | pubmed-meshheading:10082387... | lld:pubmed |
| pubmed-article:10082387 | pubmed:meshHeading | pubmed-meshheading:10082387... | lld:pubmed |
| pubmed-article:10082387 | pubmed:meshHeading | pubmed-meshheading:10082387... | lld:pubmed |
| pubmed-article:10082387 | pubmed:meshHeading | pubmed-meshheading:10082387... | lld:pubmed |
| pubmed-article:10082387 | pubmed:meshHeading | pubmed-meshheading:10082387... | lld:pubmed |
| pubmed-article:10082387 | pubmed:meshHeading | pubmed-meshheading:10082387... | lld:pubmed |
| pubmed-article:10082387 | pubmed:meshHeading | pubmed-meshheading:10082387... | lld:pubmed |
| pubmed-article:10082387 | pubmed:meshHeading | pubmed-meshheading:10082387... | lld:pubmed |
| pubmed-article:10082387 | pubmed:meshHeading | pubmed-meshheading:10082387... | lld:pubmed |
| pubmed-article:10082387 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10082387 | pubmed:articleTitle | The defective interfering particles of equine herpesvirus 1 encode an ICP22/ICP27 hybrid protein that alters viral gene regulation. | lld:pubmed |
| pubmed-article:10082387 | pubmed:affiliation | Department of Microbiology and Immunology, Louisiana State University Medical Center, Shreveport 71130-3932, USA. | lld:pubmed |
| pubmed-article:10082387 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10082387 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10082387 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10082387 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10082387 | lld:pubmed |
