| pubmed-article:10081767 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10081767 | lifeskim:mentions | umls-concept:C0080103 | lld:lifeskim |
| pubmed-article:10081767 | lifeskim:mentions | umls-concept:C0003250 | lld:lifeskim |
| pubmed-article:10081767 | lifeskim:mentions | umls-concept:C0003316 | lld:lifeskim |
| pubmed-article:10081767 | lifeskim:mentions | umls-concept:C0017837 | lld:lifeskim |
| pubmed-article:10081767 | lifeskim:mentions | umls-concept:C0020964 | lld:lifeskim |
| pubmed-article:10081767 | lifeskim:mentions | umls-concept:C0205369 | lld:lifeskim |
| pubmed-article:10081767 | lifeskim:mentions | umls-concept:C1283195 | lld:lifeskim |
| pubmed-article:10081767 | lifeskim:mentions | umls-concept:C1704788 | lld:lifeskim |
| pubmed-article:10081767 | lifeskim:mentions | umls-concept:C0321804 | lld:lifeskim |
| pubmed-article:10081767 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:10081767 | pubmed:dateCreated | 1999-5-27 | lld:pubmed |
| pubmed-article:10081767 | pubmed:abstractText | Monoclonal antibodies to the 28kDa glutathione S-transferase of Schistosoma bovis have been constructed in mice and used to characterize the epitope(s) potentially implied in the induction of anti-fecundity and anti-egg viability immune responses. Among the MoAbs produced three were particularly studied: Sb4-50 (IgG2a) and Sb4-56 (IgG1) which inhibited Sb28GST activity and Sb4-10 (IgG1) which did not. The use of overlapping peptides covering the entire amino acid sequence of Sb28GST, allowed us to define the linear epitopes recognized by these anti-Sb28GST MoAbs. Amino acid residues 202-211 were recognized by both MoAbs Sb4-50 and Sb4-56 and MoAb Sb4-10 recognized amino acid residues 58-67. Their capacity to inhibit GST activity suggested binding to the active site or to neighbouring regions, which include the C-terminal domain (a.a. 190-211) of the protein. When passively transferred into BALB/c mice MoAbs induced a significant reduction in egg hatching and an increase in immature eggs. Effects on worm burdens were, however, variable and no clear-cut association between the inhibition of enzyme activity and anti-fecundity or anti-viability activities was recorded. Our data indicate that beside the anti-fecundity and anti-viability immunity related to the impairment of GST activity, immune response to epitopes located in other regions of the molecule also contribute to the reduction of egg viability. | lld:pubmed |
| pubmed-article:10081767 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10081767 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10081767 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10081767 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10081767 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10081767 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10081767 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10081767 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10081767 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10081767 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:10081767 | pubmed:issn | 0141-9838 | lld:pubmed |
| pubmed-article:10081767 | pubmed:author | pubmed-author:FontaineJJ | lld:pubmed |
| pubmed-article:10081767 | pubmed:author | pubmed-author:CapronAA | lld:pubmed |
| pubmed-article:10081767 | pubmed:author | pubmed-author:GrzychJ MJM | lld:pubmed |
| pubmed-article:10081767 | pubmed:author | pubmed-author:Gras-MasseHH | lld:pubmed |
| pubmed-article:10081767 | pubmed:author | pubmed-author:BossusMM | lld:pubmed |
| pubmed-article:10081767 | pubmed:author | pubmed-author:LafitteSS | lld:pubmed |
| pubmed-article:10081767 | pubmed:author | pubmed-author:da CostaA VAV | lld:pubmed |
| pubmed-article:10081767 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10081767 | pubmed:volume | 21 | lld:pubmed |
| pubmed-article:10081767 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10081767 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10081767 | pubmed:pagination | 9-18 | lld:pubmed |
| pubmed-article:10081767 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:10081767 | pubmed:meshHeading | pubmed-meshheading:10081767... | lld:pubmed |
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| pubmed-article:10081767 | pubmed:meshHeading | pubmed-meshheading:10081767... | lld:pubmed |
| pubmed-article:10081767 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10081767 | pubmed:articleTitle | Definition and mapping of epitopes recognized by specific monoclonal antibodies to Schistosoma bovis 28 kDa glutathione S-transferase: relation with anti-egg viability immunity. | lld:pubmed |
| pubmed-article:10081767 | pubmed:affiliation | Centre d'Immunologie et de Biologie Parasitaire, Unité INSERM U-167, Institut Pasteur de Lille, France. | lld:pubmed |
| pubmed-article:10081767 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10081767 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
