Source:http://linkedlifedata.com/resource/pubmed/id/10076834
General Info
Affiliation
Departamento de Química Analítica, Facultad de Farmacia, Universidad de Alcalá, Alcalá de Henares, Madrid, Spain.Abstract
An amperometric immunosensor for the detection of the herbicide atrazine has been developed. A redox polymer PVPOs(bpy)2Cl was co-immobilized with the specific antibody on the surface of the electrode by crosslinking with PEGDGE to form an electron-conducting hydrogel. In a competitive assay the occurrence of the antibody-antigen reaction on the surface of the sensing film was detected through the 'electrical wiring' of the redox centres of antigen-labelled horseradish peroxidase and the electrode surface in the presence of H2O2 at 0.1 V (vsAg/AgCl).
PMID
10076834
Publication types
Research Support, Non-U.S. Gov't