Electrochemical immunosensor for the detection of atrazine.

Source:http://linkedlifedata.com/resource/pubmed/id/10076834

Download in:

View as

General Info

Authors

López MA, Ortega F, Domínguez E, Katakis I

Affiliation

Departamento de Química Analítica, Facultad de Farmacia, Universidad de Alcalá, Alcalá de Henares, Madrid, Spain.

Abstract

An amperometric immunosensor for the detection of the herbicide atrazine has been developed. A redox polymer PVPOs(bpy)2Cl was co-immobilized with the specific antibody on the surface of the electrode by crosslinking with PEGDGE to form an electron-conducting hydrogel. In a competitive assay the occurrence of the antibody-antigen reaction on the surface of the sensing film was detected through the 'electrical wiring' of the redox centres of antigen-labelled horseradish peroxidase and the electrode surface in the presence of H2O2 at 0.1 V (vsAg/AgCl).

PMID
10076834

Publication types

Research Support, Non-U.S. Gov't