| pubmed-article:10071933 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10071933 | lifeskim:mentions | umls-concept:C0315080 | lld:lifeskim |
| pubmed-article:10071933 | lifeskim:mentions | umls-concept:C0034721 | lld:lifeskim |
| pubmed-article:10071933 | lifeskim:mentions | umls-concept:C0034693 | lld:lifeskim |
| pubmed-article:10071933 | lifeskim:mentions | umls-concept:C0039067 | lld:lifeskim |
| pubmed-article:10071933 | lifeskim:mentions | umls-concept:C0040711 | lld:lifeskim |
| pubmed-article:10071933 | lifeskim:mentions | umls-concept:C0027934 | lld:lifeskim |
| pubmed-article:10071933 | lifeskim:mentions | umls-concept:C1420192 | lld:lifeskim |
| pubmed-article:10071933 | lifeskim:mentions | umls-concept:C1167622 | lld:lifeskim |
| pubmed-article:10071933 | lifeskim:mentions | umls-concept:C1533691 | lld:lifeskim |
| pubmed-article:10071933 | lifeskim:mentions | umls-concept:C0936012 | lld:lifeskim |
| pubmed-article:10071933 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:10071933 | pubmed:dateCreated | 1999-5-27 | lld:pubmed |
| pubmed-article:10071933 | pubmed:abstractText | Botulinum neurotoxins (BoNTs) are highly potent toxins that inhibit neurotransmitter release from peripheral cholinergic synapses. BoNTs consist of a toxifying light chain (LC; 50 kDa) and a binding/translocating heavy chain (HC; 100 kDa) linked through a disulfide bond. A DNA fragment encoding type A Clostridium botulinum heavy chain (BoNT/A HC) was amplified by polymerase chain reaction and cloned into an E. coli PET-15b vector. In vitro translated [35S]BoNT/A HC was identified by anti-BoNT/A polyclonal antibodies, and was used to investigate the binding of the toxin to rat synaptosomes. The binding of [35S]BoNT/A HC to synaptosomes was abolished by 500-fold excess of cold BoNT/A, and by incubation with trypsin. Treatment of BoNT/A HC with anti-BoNT/A or G(T1b) blocked its binding to synaptosomes. The radioactive BoNT/A HC recognized three proteins corresponding to a molecular mass of 150 (P150), 120 (P120), and 75 (P75) kDa in rat and bovine synaptosomal preparations. These results represent the first successful expression of functional full-length BoNT heavy chain. | lld:pubmed |
| pubmed-article:10071933 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10071933 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10071933 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10071933 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10071933 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10071933 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10071933 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10071933 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10071933 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:10071933 | pubmed:issn | 0277-8033 | lld:pubmed |
| pubmed-article:10071933 | pubmed:author | pubmed-author:SinghB RBR | lld:pubmed |
| pubmed-article:10071933 | pubmed:author | pubmed-author:LORR | lld:pubmed |
| pubmed-article:10071933 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10071933 | pubmed:volume | 18 | lld:pubmed |
| pubmed-article:10071933 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10071933 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10071933 | pubmed:pagination | 89-95 | lld:pubmed |
| pubmed-article:10071933 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
| pubmed-article:10071933 | pubmed:meshHeading | pubmed-meshheading:10071933... | lld:pubmed |
| pubmed-article:10071933 | pubmed:meshHeading | pubmed-meshheading:10071933... | lld:pubmed |
| pubmed-article:10071933 | pubmed:meshHeading | pubmed-meshheading:10071933... | lld:pubmed |
| pubmed-article:10071933 | pubmed:meshHeading | pubmed-meshheading:10071933... | lld:pubmed |
| pubmed-article:10071933 | pubmed:meshHeading | pubmed-meshheading:10071933... | lld:pubmed |
| pubmed-article:10071933 | pubmed:meshHeading | pubmed-meshheading:10071933... | lld:pubmed |
| pubmed-article:10071933 | pubmed:meshHeading | pubmed-meshheading:10071933... | lld:pubmed |
| pubmed-article:10071933 | pubmed:meshHeading | pubmed-meshheading:10071933... | lld:pubmed |
| pubmed-article:10071933 | pubmed:meshHeading | pubmed-meshheading:10071933... | lld:pubmed |
| pubmed-article:10071933 | pubmed:meshHeading | pubmed-meshheading:10071933... | lld:pubmed |
| pubmed-article:10071933 | pubmed:meshHeading | pubmed-meshheading:10071933... | lld:pubmed |
| pubmed-article:10071933 | pubmed:meshHeading | pubmed-meshheading:10071933... | lld:pubmed |
| pubmed-article:10071933 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10071933 | pubmed:articleTitle | In vitro translation of type A Clostridium botulinum neurotoxin heavy chain and analysis of its binding to rat synaptosomes. | lld:pubmed |
| pubmed-article:10071933 | pubmed:affiliation | Department of Chemistry and Biochemistry, University of Massachusetts Dartmouth, 02747, USA. | lld:pubmed |
| pubmed-article:10071933 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10071933 | pubmed:publicationType | In Vitro | lld:pubmed |
| pubmed-article:10071933 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:10071933 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10071933 | lld:pubmed |
