| pubmed-article:10048394 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10048394 | lifeskim:mentions | umls-concept:C0042774 | lld:lifeskim |
| pubmed-article:10048394 | lifeskim:mentions | umls-concept:C1707719 | lld:lifeskim |
| pubmed-article:10048394 | lifeskim:mentions | umls-concept:C0679622 | lld:lifeskim |
| pubmed-article:10048394 | lifeskim:mentions | umls-concept:C0205314 | lld:lifeskim |
| pubmed-article:10048394 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:10048394 | pubmed:dateCreated | 1999-5-13 | lld:pubmed |
| pubmed-article:10048394 | pubmed:abstractText | With the cloning of DNA encoding the trans-dominant negative mutant form of the HSV-1 origin-binding protein UL9, UL9-C535C, under the control of the tet operator-bearing hCMV major immediate-early promoter (pcmvtetO), this article demonstrates that the tetR-mediated mammalian transcription repression switch (Yao et al., Hum. Gene Ther. 9:1939-1950, 1998) can be converted to a novel HSV-1-specific viral replication switch. Using this viral replication switch, the plaque-forming efficiency of infectious HSV-1 DNA can be reversibly regulated by tetR over 100-fold in transient viral infection assays. Moreover, while less than 0 PFU/ml of HSV-1 was detected from tetR-expressing cells transfected with infectious HSV-1 DNA and plasmid pcmvtetOUL9-C535C in the presence of tetracycline, close to 1000 PFU/ml of HSV-1 was produced when similar experiments were carried out in the absence of tetracycline. The tetracycline treatment led no reduction in HSV-1 synthesis in cells transfected with infectious HSV-1 DNA alone. Taken together, given that the UL9-C535C-associated antiviral activity can be silenced in the context of this HSV-1 replication switch, the establishment of this reversible switch would allow construction of a new generation of HSV-1 recombinants able to inhibit its own replication as well as replication of wild-type virus. | lld:pubmed |
| pubmed-article:10048394 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10048394 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10048394 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10048394 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10048394 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10048394 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10048394 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10048394 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10048394 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10048394 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10048394 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10048394 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10048394 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:10048394 | pubmed:issn | 1043-0342 | lld:pubmed |
| pubmed-article:10048394 | pubmed:author | pubmed-author:ErikssonEE | lld:pubmed |
| pubmed-article:10048394 | pubmed:author | pubmed-author:YaoFF | lld:pubmed |
| pubmed-article:10048394 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10048394 | pubmed:day | 10 | lld:pubmed |
| pubmed-article:10048394 | pubmed:volume | 10 | lld:pubmed |
| pubmed-article:10048394 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10048394 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10048394 | pubmed:pagination | 419-27 | lld:pubmed |
| pubmed-article:10048394 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
| pubmed-article:10048394 | pubmed:meshHeading | pubmed-meshheading:10048394... | lld:pubmed |
| pubmed-article:10048394 | pubmed:meshHeading | pubmed-meshheading:10048394... | lld:pubmed |
| pubmed-article:10048394 | pubmed:meshHeading | pubmed-meshheading:10048394... | lld:pubmed |
| pubmed-article:10048394 | pubmed:meshHeading | pubmed-meshheading:10048394... | lld:pubmed |
| pubmed-article:10048394 | pubmed:meshHeading | pubmed-meshheading:10048394... | lld:pubmed |
| pubmed-article:10048394 | pubmed:meshHeading | pubmed-meshheading:10048394... | lld:pubmed |
| pubmed-article:10048394 | pubmed:meshHeading | pubmed-meshheading:10048394... | lld:pubmed |
| pubmed-article:10048394 | pubmed:meshHeading | pubmed-meshheading:10048394... | lld:pubmed |
| pubmed-article:10048394 | pubmed:meshHeading | pubmed-meshheading:10048394... | lld:pubmed |
| pubmed-article:10048394 | pubmed:meshHeading | pubmed-meshheading:10048394... | lld:pubmed |
| pubmed-article:10048394 | pubmed:meshHeading | pubmed-meshheading:10048394... | lld:pubmed |
| pubmed-article:10048394 | pubmed:meshHeading | pubmed-meshheading:10048394... | lld:pubmed |
| pubmed-article:10048394 | pubmed:meshHeading | pubmed-meshheading:10048394... | lld:pubmed |
| pubmed-article:10048394 | pubmed:meshHeading | pubmed-meshheading:10048394... | lld:pubmed |
| pubmed-article:10048394 | pubmed:meshHeading | pubmed-meshheading:10048394... | lld:pubmed |
| pubmed-article:10048394 | pubmed:meshHeading | pubmed-meshheading:10048394... | lld:pubmed |
| pubmed-article:10048394 | pubmed:meshHeading | pubmed-meshheading:10048394... | lld:pubmed |
| pubmed-article:10048394 | pubmed:meshHeading | pubmed-meshheading:10048394... | lld:pubmed |
| pubmed-article:10048394 | pubmed:meshHeading | pubmed-meshheading:10048394... | lld:pubmed |
| pubmed-article:10048394 | pubmed:meshHeading | pubmed-meshheading:10048394... | lld:pubmed |
| pubmed-article:10048394 | pubmed:meshHeading | pubmed-meshheading:10048394... | lld:pubmed |
| pubmed-article:10048394 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10048394 | pubmed:articleTitle | A novel tetracycline-inducible viral replication switch. | lld:pubmed |
| pubmed-article:10048394 | pubmed:affiliation | Brigham and Women's Hospital, and Department of Surgery, Harvard Medical School, Boston, MA 02115, USA. FYao@RICS.BWH.Harvard.Edu | lld:pubmed |
| pubmed-article:10048394 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10048394 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10048394 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10048394 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10048394 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10048394 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10048394 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10048394 | lld:pubmed |
