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pubmed-article:10037822pubmed:abstractTextA new method for amplifying cDNA ends is described which requires only first-strand cDNA synthesis and a single PCR to generate a correct product with very low or no background. The method can be successfully applied to total RNA as well as poly A+ RNA. The same first-strand cDNA can be used to amplify flanking sequences of any cDNA species present in the sample.lld:pubmed
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pubmed-article:10037822pubmed:articleTitleAmplification of cDNA ends based on template-switching effect and step-out PCR.lld:pubmed
pubmed-article:10037822pubmed:affiliationInstitute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, Moscow 117871, Russia.lld:pubmed
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