Statements in which the resource exists.
SubjectPredicateObjectContext
pubmed-article:10030073rdf:typepubmed:Citationlld:pubmed
pubmed-article:10030073lifeskim:mentionsumls-concept:C0007634lld:lifeskim
pubmed-article:10030073lifeskim:mentionsumls-concept:C0231441lld:lifeskim
pubmed-article:10030073lifeskim:mentionsumls-concept:C0724683lld:lifeskim
pubmed-article:10030073pubmed:issue5lld:pubmed
pubmed-article:10030073pubmed:dateCreated1999-5-4lld:pubmed
pubmed-article:10030073pubmed:abstractTextFour rabies antigen batches were produced from virus suspensions resulting from BHK21 cells adhered to microcarriers (Cytodex 1), inoculated and cultured in a bioreactor. In parallel the methodology of production of rabies virus through cultures of BHK21 cells in monolayers in bottles was used. The results obtained showed that infecting titles were 10(6.69) DL50/mL and 10(7.28) DL50/mL for suspensions cultured in bottles and in the bioreactor, respectively. The viral suspension volumes collected were on average 11,900 per batch from the bioreactor and 800mL per bottle. Ten horses were immunized with the antigen produced in the bioreactor. The means of antirabies antibody titers found were 240 and 212 IU/mL after the initial and the first booster doses, respectively. Rabies antigen with satisfactory infecting titers can be obtained on a large scale by culturing in a bioreactor inoculated BHK21 cells adhered to microcarriers.lld:pubmed
pubmed-article:10030073pubmed:languageenglld:pubmed
pubmed-article:10030073pubmed:journalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:10030073pubmed:citationSubsetIMlld:pubmed
pubmed-article:10030073pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:10030073pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:10030073pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:10030073pubmed:statusMEDLINElld:pubmed
pubmed-article:10030073pubmed:issn0036-4665lld:pubmed
pubmed-article:10030073pubmed:authorpubmed-author:GallinaN MNMlld:pubmed
pubmed-article:10030073pubmed:authorpubmed-author:FuchesR MRMlld:pubmed
pubmed-article:10030073pubmed:authorpubmed-author:FranciscoI...lld:pubmed
pubmed-article:10030073pubmed:authorpubmed-author:PaoliR de...lld:pubmed
pubmed-article:10030073pubmed:authorpubmed-author:GarciaG de...lld:pubmed
pubmed-article:10030073pubmed:issnTypePrintlld:pubmed
pubmed-article:10030073pubmed:volume40lld:pubmed
pubmed-article:10030073pubmed:ownerNLMlld:pubmed
pubmed-article:10030073pubmed:authorsCompleteYlld:pubmed
pubmed-article:10030073pubmed:pagination291-4lld:pubmed
pubmed-article:10030073pubmed:dateRevised2004-11-17lld:pubmed
pubmed-article:10030073pubmed:meshHeadingpubmed-meshheading:10030073...lld:pubmed
pubmed-article:10030073pubmed:meshHeadingpubmed-meshheading:10030073...lld:pubmed
pubmed-article:10030073pubmed:meshHeadingpubmed-meshheading:10030073...lld:pubmed
pubmed-article:10030073pubmed:meshHeadingpubmed-meshheading:10030073...lld:pubmed
pubmed-article:10030073pubmed:meshHeadingpubmed-meshheading:10030073...lld:pubmed
pubmed-article:10030073pubmed:meshHeadingpubmed-meshheading:10030073...lld:pubmed
pubmed-article:10030073pubmed:meshHeadingpubmed-meshheading:10030073...lld:pubmed
pubmed-article:10030073pubmed:meshHeadingpubmed-meshheading:10030073...lld:pubmed
pubmed-article:10030073pubmed:meshHeadingpubmed-meshheading:10030073...lld:pubmed
pubmed-article:10030073pubmed:articleTitleObtention of rabies antigen through BHK21 cells adhered to microcarriers.lld:pubmed
pubmed-article:10030073pubmed:affiliationInstituto Butantan, São Paulo, Brasil.lld:pubmed
pubmed-article:10030073pubmed:publicationTypeJournal Articlelld:pubmed