. "Reviewed: Gagneux, P, 2010-04-16"^^ . "Edited: Jassal, B, 2009-11-10"^^ . "Authored: Dall'Olio, GM, 2009-11-10"^^ . "In the first step of N-glycan precursor (LLO) synthesis, N-acetylglucosamine is added, via an alpha-1,3 linkage, to a molecule of dolichyl phosphate, producing N-acetyl-D-glucosaminyl-diphosphodolichol (Eckert V et al, 1998). This reaction is catalyzed by DPAGT1 (ALG7 in yeast), mutations in which are associated with CDG disorder type I-J (Wu X et al, 2003). The dolichyl phosphate acts as an anchor for the LLO, so the following sugar-addition reactions take place on a sugar anchored in the ER membrane."^^ . . . . . . . "Addition of N-acetyl-D-glucosamine to Dolichyl phosphate"^^ . "2.7.8.15"^^ . . . . .