When this enzyme acts on tRNA(Glu), it catalyzes the same reaction as EC 6.1.1.17.
| Predicate | Object |
|---|---|
| rdf:type | |
| rdfs:comment |
It has, however, diminished discrimination, so that it can also form glutamate-tRNA(Gln).,
The ability of this enzyme to recognize both tRNA(Glu) and one of the tRNA(Gln) isoacceptors derives from their sharing a major identity element, a hypermodified derivative of U34 (5-methylaminomethyl-2-thiouridine).,
The glutamyl-tRNA(Gln) is not used in protein synthesis until it is converted by EC 6.3.5.7 into glutaminyl-tRNA(Gln).,
This accounts for the ability of this enzyme in, for example, Bacillus subtilis, to recognize both tRNA(1)(Gln) (UUG anticodon) and tRNA(Glu) (UUC anticodon) but not tRNA(2)(Gln) (CUG anticodon).,
This relaxation of specificity has been found to result from the absence of a loop in the tRNA that specifically recognizes the third position of the anticodon.,
When this enzyme acts on tRNA(Glu), it catalyzes the same reaction as EC 6.1.1.17.
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| rdfs:subClassOf | |
| skos:broaderTransitive | |
| uniprot:name |
Glutamate--tRNA(Gln) ligase,
Nondiscriminating glutamyl-tRNA synthetase
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| uniprot:activity |
ATP + L-glutamate + tRNA(Glx) = AMP + diphosphate + glutamyl-tRNA(Glx).
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