We have analyzed the immune system in Stat5-deficient mice. Although Stat5a-/-splenocytes have a partial defect in anti-CD3-induced proliferation that can be overcome by high dose interleukin (IL)-2, we now demonstrate that defective proliferation in Stat5b-/-splenocytes cannot be corrected by this treatment. Interestingly, this finding may be at least partially explained by diminished expression of the IL-2 receptor beta chain (IL-2Rbeta), which is a component of the receptors for both IL-2 and IL-15, although other defects may also exist. Similar to the defect in proliferation in activated splenocytes, freshly isolated splenocytes from Stat5b-/-mice exhibited greatly diminished proliferation in response to IL-2 and IL-15. This results from both a decrease in the number and responsiveness of natural killer (NK) cells. Corresponding to the diminished proliferation, basal as well as IL-2- and IL-15-mediated boosting of NK cytolytic activity was also greatly diminished. These data indicate an essential nonredundant role for Stat5b for potent NK cell-mediated proliferation and cytolytic activity.
| Predicate | Object |
|---|---|
| rdf:type | |
| rdfs:comment |
We have analyzed the immune system in Stat5-deficient mice. Although Stat5a-/-splenocytes have a partial defect in anti-CD3-induced proliferation that can be overcome by high dose interleukin (IL)-2, we now demonstrate that defective proliferation in Stat5b-/-splenocytes cannot be corrected by this treatment. Interestingly, this finding may be at least partially explained by diminished expression of the IL-2 receptor beta chain (IL-2Rbeta), which is a component of the receptors for both IL-2 and IL-15, although other defects may also exist. Similar to the defect in proliferation in activated splenocytes, freshly isolated splenocytes from Stat5b-/-mice exhibited greatly diminished proliferation in response to IL-2 and IL-15. This results from both a decrease in the number and responsiveness of natural killer (NK) cells. Corresponding to the diminished proliferation, basal as well as IL-2- and IL-15-mediated boosting of NK cytolytic activity was also greatly diminished. These data indicate an essential nonredundant role for Stat5b for potent NK cell-mediated proliferation and cytolytic activity.
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| skos:exactMatch | |
| uniprot:name |
J. Exp. Med.
|
| uniprot:author |
Bloom E.T.,
Davey H.W.,
Horvath-Arcidiacono J.A.,
Imada K.,
Leonard W.J.,
Nakajima H.,
Udy G.B.
|
| uniprot:date |
1998
|
| uniprot:pages |
2067-2074
|
| uniprot:title |
Stat5b is essential for natural killer cell-mediated proliferation and cytolytic activity.
|
| uniprot:volume |
188
|
| dc-term:identifier |
doi:10.1084/jem.188.11.2067
|