The crystal structure of acidic phospholipase A2 from the venom of Agkistrodon halys pallas has been determined by molecular replacement at 2.0 A resolution to a crystallographic R-factor of 0.157. The overall structure of the molecule is very similar to those of other phospholipase A2 species of known structure. The catalytic site, the hydrophobic channel and the N-terminal region show greatest structural conservation. The Ca(2+)-binding region has a conformation that resembles closely that of bovine PLA2 rather than Crotalus atrox PLA2. Compared with other PLA2 species, the conformation of the C-terminal ridge shows significant difference due to the insertion of two residues. A unique aromatic patch appears on one face of the molecules, surrounded by two acidic residues, the relevant features of this structure and their possible biological implications are discussed.
| Predicate | Object |
|---|---|
| rdf:type | |
| rdfs:comment |
The crystal structure of acidic phospholipase A2 from the venom of Agkistrodon halys pallas has been determined by molecular replacement at 2.0 A resolution to a crystallographic R-factor of 0.157. The overall structure of the molecule is very similar to those of other phospholipase A2 species of known structure. The catalytic site, the hydrophobic channel and the N-terminal region show greatest structural conservation. The Ca(2+)-binding region has a conformation that resembles closely that of bovine PLA2 rather than Crotalus atrox PLA2. Compared with other PLA2 species, the conformation of the C-terminal ridge shows significant difference due to the insertion of two residues. A unique aromatic patch appears on one face of the molecules, surrounded by two acidic residues, the relevant features of this structure and their possible biological implications are discussed.
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| skos:exactMatch | |
| uniprot:name |
J. Mol. Biol.
|
| uniprot:author |
Chen Y.-C.,
Gui L.-L.,
Lin Z.-J.,
Wang X.-Q.,
Yang J.,
Zhou Y.-C.
|
| uniprot:date |
1996
|
| uniprot:pages |
669-676
|
| uniprot:title |
Crystal structure of an acidic phospholipase A2 from the venom of Agkistrodon halys pallas at 2.0-A resolution.
|
| uniprot:volume |
255
|
| dc-term:identifier |
doi:10.1006/jmbi.1996.0054
|