A gene encoding a chitosanase (mschito) was cloned from Microbacterium sp. OU01. The ORF consists of 801 bp which encoded a polypeptide of 266 amino acid residues. The deduced amino acid sequence shows 98% identity to that of the chitosanase reported in Pseudomonas sp. A-01. In addition, the fusion protein containing MSCHITO was expressed in E. coli and purified using Ni-NTA affinity chromatography. The purified rMSCHITO protein degraded the chitosan (the degree of deacetylation of 99%) and produced a mixture of chitooligosaccharides. The MSCHITO is thus an endo-chitosanase.
| Predicate | Object |
|---|---|
| rdf:type | |
| rdfs:comment |
A gene encoding a chitosanase (mschito) was cloned from Microbacterium sp. OU01. The ORF consists of 801 bp which encoded a polypeptide of 266 amino acid residues. The deduced amino acid sequence shows 98% identity to that of the chitosanase reported in Pseudomonas sp. A-01. In addition, the fusion protein containing MSCHITO was expressed in E. coli and purified using Ni-NTA affinity chromatography. The purified rMSCHITO protein degraded the chitosan (the degree of deacetylation of 99%) and produced a mixture of chitooligosaccharides. The MSCHITO is thus an endo-chitosanase.
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| skos:exactMatch | |
| uniprot:name |
Biotechnol. Lett.
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| uniprot:author |
Sun Y.,
Zhang J.
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| uniprot:date |
2007
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| uniprot:pages |
1221-1225
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| uniprot:title |
Molecular cloning, expression and characterization of a chitosanase from Microbacterium sp.
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| uniprot:volume |
29
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| dc-term:identifier |
doi:10.1007/s10529-007-9373-y
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