_1312751288758863440

1 last-imex-assigned, 2007 publication year MI:0886, Apoptosis - Interactions involving proteins with a function related to apoptosis dataset MI:0875, Chemical crosslinking reagent dithiobis (succinimidyl propionate) (DSP) was administered to rat vascular endothelial cells (1 mM for 30 min) following a 30 min incubation with preferentially biotinylated rat recombinant TNF-alpha. Following crosslinking and cell lysis, the biotinylated signaling complex was recovered using immobilized streptavidin. The tryptic digest mixture was analyzed via LC MS/MS. exp-modification MI:0627, Freed JK., Smith JR., Li P., Greene AS. author-list MI:0636, Isolation of signal transduction complexes using biotin and crosslinking methodologies., Only protein-protein interactions full coverage MI:0957, Protein mapping from IPI identifiers to UniProt KB accession numbers was carried out using the PICR tool (http://www.ebi.ac.uk/Tools/picr).Mappings are done on the basis of 100% sequence identity over the whole sequence. The following IPI numbers were not valid IPI00199003, IPI00009865 and IPI00372477. The following proteins could not be mapped to UniProt accession numbers and are identified by IntAct identifiers (bracketed identifier) ; IPI00373233 (EBI-1571381), IPI00365192 (EBI-1571384), IPI00365130 (EBI-1571388), IPI00372079 (EBI-1571392), IPI00368391 (EBI-1572037), IPI00767320 (EBI-1572050), IPI00367347 (EBI-1572054), IPI00361486 (EBI-1572056), IPI00368082 (EBI-1572059), IPI00370353 (EBI-1572062), IPI00766216 (EBI-1572065), IPI00767314 (EBI-1572068), IPI00360086 (EBI-1572071), IPI00767813 (EBI-1572071), IPI00768298 (EBI-1572074), IPI00768616 (EBI-1572077), IPI00391468 (EBI-1572080), IPI00768003 (EBI-1572083), IPI00767360 (EBI-1572086), IPI00767825 (EBI-1572089), IPI00764166 (EBI-1572092), IPI00188079 (EBI-1572095), IPI00368692 (EBI-1572098), IPI00763922 (EBI-1572101) and IPI00203773 (EBI-1572104). data-processing MI:0633, Proteomics (1615-9853) journal MI:0885, Synapse - Interactions of proteins with an established role in the presynapse. dataset MI:0875, The authors used the following critrea to select identified proteins. The proteins had to be identified in at least two out of four separate MS/MS runs; the protein had to be identified using at least five peptides; at least one of the peptides used to identify the protein had to have a peptide score of >=0.5 (Mascot search engine score). comment MI:0612, imex curation MI:0959, imex curation curation depth MI:0955

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